Lycopene ameliorates insulin resistance and increases muscle capillary density in aging via activation of SIRT1
Lycopene ameliorates insulin resistance and increases muscle capillary density in aging via activation of SIRT1
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DOI：
10.1016/j.jnutbio.2021.108862
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发表时间：
2021-10-08
影响因子：
5.6
通讯作者：
Yang, Xiaolong
中科院分区：
文献类型：
--
作者：
Li, Jing;Zhang, Yingjiang;Yang, Xiaolong
番茄红素（Ly）是一种碳氢化合物，属于四萜类胡萝卜素家族，存在于红色水果和蔬菜中。毛细血管密度和血流量随年龄增长而降低是导致死亡率和发病率增加的重要原因。本研究旨在探讨生物活性食品Ly对血管衰老的影响及其可能机制。细胞学结果表明，赖氨酸能促进人脐静脉内皮细胞（HUVECs）增殖，增强HUVECs形成毛细血管样结构的能力。SIRT 1在衰老HUVECs中表达上调。在体内，衰老的大鼠表现出胰岛素抵抗和血管损伤的迹象。此外，在D-半乳糖诱导的和自然老化的肌肉中，毛细血管密度和血流量在血管老化过程中减少。然而，当给予Ly时，这些条件可以逆转。同时测定了ATP、乳酸和甘草酸的含量，发现Ly可通过提高葡萄糖的利用率，促进能量代谢，从而促进血管生成。最后，在胰岛素抵抗细胞模型中，我们敲除SIRT 1后给予赖氨酸，发现其不能恢复胰岛素的转导。总之，本研究中的所有数据表明，Ly可以重新激活SIRT 1并改善胰岛素抵抗，这是血管老化的可逆原因。(c)2021年由Elsevier Inc.出版
Lycopene (Ly) is a kind of hydrocarbon, which belongs to the family of tetraterpene carotene and exists in red fruits and vegetables. The decrease of capillary density and blood flow with age is a significant reason for the increase of mortality and morbidity. Herein, our study aims to explore the effects of Ly (a bioactive food compound) on vascular aging in vitro and in vivo and its potential mechanisms. The cytological results showed that Ly could promote the proliferation of human umbilical vein endothelial cell (HUVECs) and enhance the ability of HUVECs to form capillary-like structures. Furthermore, the expression of SIRT1 in aged HUVECs was up-regulated. In vivo , aging rats showed signs of insulin resistance and blood vessel damage. Additionally, the capillary density and blood flow were reduced during the vascular aging process in both D-gal-induced and naturally aging muscle. However, when Ly was given, these conditions could be reversed. Simultaneously, the contents of ATP, lactic acid and pyruvic acid were determined, and it was found that Ly could promote angiogenesis by increasing the utilization rate of glucose and promoting energy metabolism. Finally, in the insulin resistance cell model, we knocked down the SIRT1 and administrated with Ly, and found that it couldn't restore insulin transdution. In conclusion, all the data in this study demonstrate that Ly could reactivate SIRT1 and improve insulin resistance, which was a reversible cause of vascular aging. (c) 2021 Published by Elsevier Inc.