A rat model for muscle regeneration in the soft palate.
A rat model for muscle regeneration in the soft palate.
复制标题
软pa的大鼠肌肉再生模型。
DOI：
10.1371/journal.pone.0059193
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wagener FA
中科院分区：
文献类型：
--
作者：
Carvajal Monroy PL;Grefte S;Kuijpers-Jagtman AM;Helmich MP;Ulrich DJ;Von den Hoff JW;Wagener FA
患有软腭裂的儿童在言语、吞咽和吮吸方面有困难。尽管手术成功地重新定位肌肉，但通常无法实现最佳功能。腭裂修复后瘢痕形成和再生缺陷可能会阻碍肌肉功能的恢复。因此，本研究的目的是研究大鼠软腭的解剖学和组织学，并建立一个手术损伤后肌肉再生的体内模型。将14只成年雄性Sprague道利大鼠分为4组。第1组（n = 4）和第2组（n = 2）分别用于研究软腭的解剖学和组织学。 第3组（n = 6）用于软腭手术创伤，第4组（n = 2）用作未创伤对照组。 7天后通过（免疫）组织化学（AZAN染色、Pax7、MyoD、MyoG、MyHC和ASMA）评价伤口（1 mm）。本研究表明，大鼠软腭肌肉的解剖学和组织学在很大程度上与人类相似。所有伤口在7天后显示出愈合的临床证据。AZAN染色显示在创伤区域中广泛的胶原沉积，以及肌纤维和唾液腺的初始再生。通过抗体染色鉴定创伤区域中的增殖和分化卫星细胞。该模型是第一个适合研究大鼠软腭肌肉再生的模型，并允许开发新的辅助策略来促进腭裂手术后的肌肉再生。
Children with a cleft in the soft palate have difficulties with speech, swallowing, and sucking. Despite successful surgical repositioning of the muscles, optimal function is often not achieved. Scar formation and defective regeneration may hamper the functional recovery of the muscles after cleft palate repair. Therefore, the aim of this study is to investigate the anatomy and histology of the soft palate in rats, and to establish an in vivo model for muscle regeneration after surgical injury. Fourteen adult male Sprague Dawley rats were divided into four groups. Groups 1 (n = 4) and 2 (n = 2) were used to investigate the anatomy and histology of the soft palate, respectively. Group 3 (n = 6) was used for surgical wounding of the soft palate, and group 4 (n = 2) was used as unwounded control group. The wounds (1 mm) were evaluated by (immuno)histochemistry (AZAN staining, Pax7, MyoD, MyoG, MyHC, and ASMA) after 7 days. The present study shows that the anatomy and histology of the soft palate muscles of the rat is largely comparable with that in humans. All wounds showed clinical evidence of healing after 7 days. AZAN staining demonstrated extensive collagen deposition in the wound area, and initial regeneration of muscle fibers and salivary glands. Proliferating and differentiating satellite cells were identified in the wound area by antibody staining. This model is the first, suitable for studying muscle regeneration in the rat soft palate, and allows the development of novel adjuvant strategies to promote muscle regeneration after cleft palate surgery.
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影响因子：
4.6
作者：
Lepper, Christoph;Partridge, Terence A.;Fan, Chen-Ming
通讯作者：
Fan, Chen-Ming
影响因子：
--
作者：
ALBIIN, N;HELLSTROM, S;SODERBERG, O
通讯作者：
SODERBERG, O
DOI：
10.1016/j.bjps.2005.05.019
发表时间：
2005-12-01
期刊：
BRITISH JOURNAL OF PLASTIC SURGERY
影响因子：
--
作者：
Inman, DS;Thomas, P;Reid, CA
通讯作者：
Reid, CA
影响因子：
3.6
作者：
Huang, MHS;Lee, ST;Rajendran, K
通讯作者：
Rajendran, K
DOI：
10.1083/jcb.132.4.657
发表时间：
1996-02
期刊：
The Journal of cell biology
影响因子：
--
作者：
Andrés V;Walsh K
通讯作者：
Walsh K