Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome.
Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome.
复制标题
DOI：
10.1093/dnares/2.4.153
复制
发表时间：
1995-08-31
期刊：
影响因子：
--
通讯作者：
Tabata, S
中科院分区：
文献类型：
--
作者：
Kaneko, T;Tanaka, A;Tabata, S
1,003,450 bp 的连续序列跨越集胞藻基因组的图谱位置 64% 至 92%。已推导出菌株PCC6803。对序列的计算机分析预测，该区域至少包含 818 个潜在的 ORF，其中 255 个（31%）是已识别的基因或其同源物，84 个（10%）是与注册的假设基因同源，149 个（18%）与报道的基因表现出弱相似性。其余 330 个 ORF 与任何报道的基因没有明显的相似性，或者不携带显着的蛋白质基序。潜在的ORF作为一个整体占据了测序区域的86%，这意味着基因组中基因的排列紧凑。在结构RNA基因方面，鉴定出1个由5028bp组成的rRNA操纵子和至少11种tRNA基因。值得注意的是，11 种 tRNA 中的 10 种与植物叶绿体中报道的 tRNA 表现出显着的序列相似性。与其他值得注意的独特序列一样，鉴定出了三类 IS 样元件，每种元件都具有 IS 元件的典型特征，并且在位于图谱位置 69% 的 5,079 bp 大 ORF 中鉴定出了仅在真核生物调节蛋白中检测到的典型 WD(Trp-Asp) 重复单元。
The contiguous sequence of 1,003,450 bp spanning map positions 64% to 92% of the genome of Synechocystis sp. strain PCC6803 has been deduced. Computer analysis of the sequence predicts that this region contains at least 818 potential ORFs, in which 255 (31%) were either genes that had already been identified or their homologues, 84 (10%) were homologues to registered hypothetical genes, and 149 (18%) showed weak similarities to reported genes. The remaining 330 ORFs showed no apparent similarity to any reported genes or carried no significant protein motifs. The potential ORFs as a whole occupied 86% of the sequenced region, implying compact arrangement of genes in the genome. As to the structural RNA genes, one rRNA operon consisting of 5,028 bp and at least 11 species of tRNA genes were identified. It is noteworthy that 10 out of the 11 tRNA species showed significant sequence similarities to tRNAs reported in plant chloroplasts. As other notable unique sequences, three classes of IS-like elements each with characteristics typical of IS elements were identified, and a typical unit of WD(Trp-Asp)-repeats which have only been detected in the regulatory proteins of eukaryotes was identified within the large 5,079-bp ORF located at map position 69%.