Development of a high density integrated reference genetic linkage map for the multinational Brassica rapa Genome Sequencing Project
Development of a high density integrated reference genetic linkage map for the multinational Brassica rapa Genome Sequencing Project
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DOI：
10.1139/g10-054
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发表时间：
2010-11-01
期刊：
影响因子：
3.1
通讯作者：
Lim, Yong Pyo
中科院分区：
文献类型：
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作者：
Li, Xiaonan;Ramchiary, Nirala;Lim, Yong Pyo
将78个来自Chiifu-401-42 × Kenshin（CKDH）的加倍单倍体系的参考遗传图谱和190个来自Chiifu-401-42 ×快速循环B的F-2系的新图谱结合起来，构建了一个高密度芜菁整合连锁图谱。rapa（CRF2）。该图谱包含1017个标记，覆盖B区1262.0 cM。rapa基因组的平均基因座间距离为1.24 cM。连锁群间标记顺序和位置的相似性较高，近距离倒位较少。共定位了155个SSR标记，锚定在102个新的细菌人工染色体（BAC）和146个内含子多态性（IP）标记上，这将有助于正在进行的跨国芜菁基因组测序计划（BrGSP）中测序的BAC的比对。此外，比较了B.与10个B的rapa共识图。利用98个IP标记构建的芥菜A基因组连锁群，除少数标记显示倒位或易位外，其余标记间均表现出高度共线性。这表明染色体在这些芸苔属物种之间是高度保守的，尽管它们在分化后独立进化。来自BrGSP的序列信息将对B有用。芥菜育种以及所鉴定的拟南芥染色体区块和B的已知数量性状基因座（QTL）信息。芥菜可用于改良包括B在内的其他芸苔属作物。拉帕。
We constructed a high-density Brassica rapa integrated linkage map by combining a reference genetic map of 78 doubled haploid lines derived from Chiifu-401-42 x Kenshin (CKDH) and a new map of 190 F-2 lines derived from Chiifu-401-42 x rapid cycling B. rapa (CRF2). The integrated map contains 1017 markers and covers 1262.0 cM of the B. rapa genome, with an average interlocus distance of 1.24 cM. High similarity of marker order and position was observed among the linkage groups of the maps with few short-distance inversions. In total, 155 simple sequence repeat (SSR) markers, anchored to 102 new bacterial artificial chromosomes (BACs) and 146 intron polymorphic (IP) markers were mapped in the integrated map, which would be helpful to align the sequenced BACs in the ongoing multinational Brassica rapa Genome Sequencing Project (BrGSP). Further, comparison of the B. rapa consensus map with the 10 B. juncea A-genome linkage groups by using 98 common IP markers showed high-degree colinearity between the A-genome linkage groups, except for few markers showing inversion or translocation. Suggesting that chromosomes are highly conserved between these Brassica species, although they evolved independently after divergence. The sequence information coming out of BrGSP would be useful for B. juncea breeding. and the identified Arabidopsis chromosomal blocks and known quantitative trait loci (QTL) information of B. juncea could be applied to improve other Brassica crops including B. rapa.