DIEL AND DEPTH VARIATIONS IN DISSOLVED FREE AMINO-ACIDS AND AMMONIUM IN THE BALTIC SEA DETERMINED BY SHIPBOARD HPLC ANALYSIS
DIEL AND DEPTH VARIATIONS IN DISSOLVED FREE AMINO-ACIDS AND AMMONIUM IN THE BALTIC SEA DETERMINED BY SHIPBOARD HPLC ANALYSIS
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DOI：
10.4319/lo.1982.27.2.0336
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发表时间：
1982-01-01
影响因子：
4.5
通讯作者：
LINDROTH, P
中科院分区：
文献类型：
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作者：
MOPPER, K;LINDROTH, P
采用柱前荧光标记法，对天然沃茨中溶解性游离氨基酸（DFAA）进行了船载高效液相色谱（HPLC）分析。不需要样品制备和所有天然存在的氨基酸[AA]加上NH 4+在30分钟内充分分离。观察到至少60米的深度，最大DFAA浓度（200-400 nM）在傍晚和最小浓度（30-40 nM）在白天早些时候惊人的昼夜趋势。与晚上浓度最大值同时升高的碱性氨基酸（特别是鸟氨酸和赖氨酸）和NH 4+的相对丰度。在白天，在上层水中发现了非常低水平的氨基酸。总DFAA的夜间至日间下降的幅度表明高达6 μ g AA的净异养吸收速率。l-1. cntdot. h-1。一个显着的氨基酸最大值在O2-HS-在水柱中的界面推测是来自居住在这个地区的微生物种群。明显的，巧合的最小值氨基酸和NH 4+浓度刚好在这个界面上可能与通过沉淀金属氢氧化物或增强异养使用的清除有关。
A precolumn fluorimetric labeling method was tested for shipboard HPLC [high pressure liquid chromatography] analysis of dissolved free amino acids (DFAA) in natural waters. No sample preparation is required and all naturally occurring amino acids [AA] plus NH4+ are sufficiently separated within 30 min. Striking diel trends were observed to a depth of at least 60 m, with maximal DFAA concentrations (200-400 nM) in the late evening and minimal concentrations (30-40 nM) in the early day. Concurrent with the evening concentration maxima were elevated relative abundances of basic amino acids (especially ornithine and lysine) and NH4+. During the day very low levels of amino acids were found in the upper water. The magnitude of the night-to-day drop in total DFAA suggests a net heterotrophic uptake rate of up to 6 .mu.g AA .cntdot. l-1 .cntdot. h-1. A pronounced amino acid maximum at the O2-HS- interface in the water column was presumably derived from microbial populations inhabiting this region. Pronounced, coincidental minima in amino acid and NH4+ concentrations just above this interface may be related either to scavaging by precipitating metal oxyhydroxides or to enhanced heterotrophic use.