Regional metabolic status of the E-18 rat fetal brain following transient hypoxia/ischemia.
Regional metabolic status of the E-18 rat fetal brain following transient hypoxia/ischemia.
复制标题
短暂缺氧/缺血后 E-18 大鼠胎脑的区域代谢状态。
DOI：
10.1007/s11011-006-9031-4
复制
发表时间：
2006
影响因子：
3.6
通讯作者：
Selman,WarrenR
中科院分区：
文献类型：
--
作者：
Pundik,Svetlana;Robinson,Shenandoah;Lust,WDavid;Zechel,Jennifer;Buczek,Marek;Selman,WarrenR
越来越多的证据表明，胎儿代谢应激可能会导致大脑发育过程中的各种产后扰动。该研究的目的是确定缺氧/缺血的持续时间，这将引起胎儿大脑中代谢的明显区域抑制，并进一步检查几个终点，以确定代谢应激是否影响发育中的大脑。子宫动脉和卵巢动脉的子宫分支用动脉瘤夹闭塞45 min，取出动脉瘤夹，并在回流0、2和6 h测量围产期脑的5个区域中的代谢物。局部P-肌酸、ATP和葡萄糖水平在45分钟闭塞结束时显著耗尽。糖原和谷氨酸水平在闭塞结束时显示了下降的趋势，但不显着。柠檬酸盐浓度在闭塞结束时基本保持不变。为了确保损伤对组织不致命，在再灌注2和6小时时检查代谢物的恢复，并且在所检查的脑的五个区域中，在再灌注6小时时，高能磷酸盐和葡萄糖的浓度通常是正常或接近正常的。代谢物的变化表明，45分钟的缺氧/缺血是一个合适的模型，用于研究胎儿代谢应激后的新生儿发育。
Increasing evidence indicates that fetal metabolic stress may result in a variety of post–natal perturbations during brain development. The goal of the study was to determine the duration of hypoxia/ischemia that would elicit a demonstrable regional depression of metabolism in the fetal brain and further to examine several end–points to determine if the metabolic stress affects the developing brain. The uterine artery and uterine branch of the ovarian artery were occluded with aneurysm clamps for a period of 45 min, the clips removed and the metabolites in five regions of the perinatal brain were measured at 0, 2 and 6 h of reflow. Regional P–creatine, ATP and glucose levels were significantly depleted at the end of the 45 min occlusion. The levels of glycogen and glutamate at the end of the occlusion indicated a decreasing trend which was not significant. The concentration of citrate remained essentially unchanged at the end of the occlusion. To ensure that the insult was not lethal to the tissue, the recovery of the metabolites was examined at 2 and 6 h of reflow and generally the concentrations of the high–energy phosphates and glucose were normal or near–normal by 6 h of reperfusion in the five regions of the brain examined. The changes in the metabolites indicate that 45 min of hypoxia/ischemia is an appropriate model for studying neonatal development after fetal metabolic stress.