Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds
Mesenchymal stem cell ingrowth and differentiation on coralline hydroxyapatite scaffolds
复制标题
DOI：
10.1016/j.biomaterials.2006.10.003
复制
发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Bunger, Cody
中科院分区：
文献类型：
--
作者：
Mygind, Tina;Stiehler, Maik;Bunger, Cody
在多孔支架上培养成骨细胞可以为使用患者自体人类间充质干细胞（hMSC）进行骨移植提供新的解决方案。我们比较了孔径为 200 和 500 μm 的珊瑚羟基磷灰石支架用于 hMSC 的扩增和分化。我们将 hMSC 静态培养或在旋转烧瓶中培养 1、7、14 和 21 天，通过碱性磷酸酶活性测定和 10 种成骨标志物的实时逆转录酶聚合酶链反应显示，200 μm 孔支架比 500 μm 孔支架表现出更快的成骨分化速率。 500微米的支架具有更高的增殖率并容纳更多的细胞（通过DNA含量、扫描电子显微镜和荧光显微镜显示）。因此，3D微孔生物材料的孔隙率可用于将hMSC引导至特定方向。我们发现 hMSC/支架结构的动态转瓶培养导致支架中细胞的增殖、分化和分布增加。因此，转瓶培养是一种易于使用且廉价的系统，用于在中小型 3D 支架上培养 hMSC。 (c) 2006 Elsevier Ltd. 保留所有权利。
Culture of osteogenic cells on a porous scaffold could offer a new solution to bone grafting using autologous human mesenchymal stem cells (hMSC) from the patient. We compared coralline hydroxyapatite scaffolds with pore sizes of 200 and 500 mu m for expansion and differentiation of hMSCs. We cultivated the hMSC statically or in spinner flasks for 1, 7, 14 and 21 days and found that the 200-mu m pore scaffolds exhibited a faster rate of osteogenic differentiation than did the 500-mu m pore scaffolds as shown by an alkaline phosphatase activity assay and real-time reverse transcriptase polymerase chain reaction for 10 osteogenic markers. The 500-mu m scaffolds had increased proliferation rates and accommodated a higher number of cells (shown by DNA content, scanning electron microscopy and fluorescence microscopy). Thus the porosity of a 3D microporous biomaterial may be used to steer hMSC in a particular direction. We found that dynamic spinner flask cultivation of hMSC/scaffold constructs resulted in increased proliferation, differentiation and distribution of cells in scaffolds. Therefore, spinner flask cultivation is an easy-to-use inexpensive system for cultivating hMSCs on small to intermediate size 3D scaffolds. (c) 2006 Elsevier Ltd. All rights reserved.