Developmental features of human striatal tissue transplanted in a rat model of Huntington's disease
Developmental features of human striatal tissue transplanted in a rat model of Huntington's disease
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DOI：
10.1006/nbdi.1996.0124
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发表时间：
1997-01-01
影响因子：
6.1
通讯作者：
Brundin, P
中科院分区：
文献类型：
--
作者：
GrasbonFrodl, EM;Nakao, N;Brundin, P
含有纹状体特有神经元的纹状体移植物区域被称为P区。我们研究了在亨廷顿病大鼠模型中，人外侧神经节隆起(LGE)组织异种移植中P区的缺乏是由于(I)缺乏适当的LGE神经元的靶细胞，还是(Ii)由于持续存在不成熟的形态。将不同大小(21 mm、24 mm和30 mm)的人胚胎纹状体组织移植到免疫抑制大鼠的ibotenate损毁纹状体内，15-17周后处死。在大多数情况下，来自LGE和内侧神经节隆起(MGE)的组织被一起移植，而一些大鼠仅接受LGE组织的移植，两种类型的移植物都显示出增殖细胞(增殖细胞)、Vimentin(未成熟星形胶质细胞)和GAP-43(长出的纤维)的免疫阳性染色，这表明移植物的成熟直到移植后4个月仍在进行。MGE与LGE共移植时移植物存活较好，提示MGE可能为LGE神经元提供营养支持，并可影响人纹状体异种移植物的整体存活。然而，LGE+MGE混合移植物的P区形成程度并未增加。(C)1997年学术出版社。
Areas of striatal grafts which contain neurons that are characteristic of the striatum are called P-zones. We have investigated whether the paucity of P-zones in human xenografts of lateral ganglionic eminence (LGE) tissue in a rat model of Huntington's disease is due (i) to an absence of the appropriate target cells of LGE neurons or (ii) to the persistence of an immature morphology. Striatal tissue from human embryos of varying sizes (21, 24, and 30 mm in crown-to-rump length) was grafted into the ibotenate-lesioned striatum of immunosuppressed rats, which were killed after 15-17 weeks. In most cases, tissue from the LGE and medial ganglionic eminence (MGE) was transplanted together, whereas some rats received grafts of only LGE tissue, Both types of grafts exhibited positive immunostaining for PCNA (proliferating cells), Vimentin (immature astrocytes), and GAP-43 (outgrowing fibers), which indicates that graft maturation is still ongoing up to 4 months after grafting. Graft survival seemed better when MGE was cografted with LGE, suggesting that the MGE may provide trophic support for LGE neurons and can affect the overall survival of human striatal xenografts. However, the extent of P-zone formation was not increased in MIXED, i.e., LGE plus MGE, grafts. (C) 1997 Academic Press.