Inhibition of oxidation of low density lipoprotein by vitamin E and related compounds.
Inhibition of oxidation of low density lipoprotein by vitamin E and related compounds.
复制标题
维生素 E 和相关化合物抑制低密度脂蛋白的氧化。
作者：
N. Gotoh;N. Noguchi;J. Tsuchiya;K. Morita;H. Sakai;H. Shimasaki;E. Niki
为了明确α-生育酚的抗氧化作用，进行了低密度脂蛋白（LDL）的氧化。亲脂性和亲水性偶氮化合物和铜诱导氧化的LDL类似地得到胆固醇酯和磷脂酰胆碱氢过氧化物作为主要产物。α-生育酚在LDL中的抗氧化效力比在均匀溶液中差得多。Doxyl硬脂酸被用作自旋探针并掺入LDL中。抗坏血酸盐降低LDL中的Doxyl nitroxide的速率随着与LDL表面距离的增加而降低。从自旋探针与α-生育酚之间清除自由基的竞争中发现，随着自由基深入LDL内部，α-生育酚清除自由基的效力变小。另一方面，2，2，5，7，8-五甲基-6-色醛不管氮氧自由基的位置如何都不影响自旋标记。色原烷醇对LDL氧化的抗氧化活性随着2-位类异戊二烯侧链长度的减小而增加。所有这些结果都解释了LDL中α-生育酚的位置和低迁移率。生育酚介导的繁殖在低速率的自由基通量下被观察到，但这被还原剂如抗坏血酸和泛醇抑制。
The oxidation of low density lipoprotein (LDL) was carried out aiming specifically at elucidating the anti-oxidant action of alpha-tocopherol. Lipophilic and hydrophilic azo compounds and copper induced the oxidation of LDL similarly to give cholesterol ester and phosphatidylcholine hydroperoxides as major products. The antioxidant potency of alpha-tocopherol in LDL was much poorer than in homogeneous solution. Doxyl stearic acids were used as spin probe and incorporated in LDL. The rate of reduction of doxyl nitroxide in LDL by ascorbate decreased with increasing distance from the LDL surface. From the competition between the spin probe and alpha-tocopherol in scavenging radical, it was found that the efficacy of radical scavenging by alpha-tocopherol became smaller as the radical went deeper into the interior of LDL. On the other hand, 2,2,5,7,8-pentamethyl-6-chromal spared the spin label regardless of the position of nitroxide. The antioxidant activity of chromanols against LDL oxidation increased with decreasing length of isoprenoid side chain at the 2-position. All these results were interpreted by location and low mobility of alpha-tocopherol in LDL. The tocopherol mediated propagation was observed notably at low rate of radical flux, but this was suppressed by reductant such as ascorbic acid and ubiquinol.
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影响因子：
6.5
作者：
V. Kagan;E. Serbinova;T. Forte;G. Scita;L. Packer
通讯作者：
V. Kagan;E. Serbinova;T. Forte;G. Scita;L. Packer
DOI：
10.1073/pnas.84.9.2995
发表时间：
1987-05-01
影响因子：
11.1
作者：
QUINN, MT;PARTHASARATHY, S;STEINBERG, D
通讯作者：
STEINBERG, D
DOI：
10.1097/00006205-199307000-00001
发表时间：
1993-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
M. Stampfer;C. Hennekens;J. Manson;G. Colditz;B. Rosner;W. Willett
通讯作者：
M. Stampfer;C. Hennekens;J. Manson;G. Colditz;B. Rosner;W. Willett
DOI：
10.1073/pnas.81.12.3883
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
STEINBRECHER, UP;PARTHASARATHY, S;STEINBERG, D
通讯作者：
STEINBERG, D
影响因子：
56.9
作者：
HABERLAND, ME;FONG, D;CHENG, L
通讯作者：
CHENG, L