SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae
SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae
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DOI：
10.1101/gad.1430406
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发表时间：
2006-08-01
影响因子：
10.5
通讯作者：
Wang, Ting-Fang
中科院分区：
文献类型：
--
作者：
Cheng, Chung-Hsu;Lo, Yu-Hui;Wang, Ting-Fang
联会复合体（SC）是一种蛋白质复合体，在减数分裂前期介导同源染色体间的联会。在酿酒酵母中，Zip1蛋白是SC的组成部分。在没有DNA双链断裂或SC起始蛋白Zip3的情况下，Zip1蛋白聚集形成多复合物（PC）。此外，Zip1还负责在减数分裂前期早期的DSB独立的非同源着丝粒偶联。我们在这里报告说，Zip3是一个SUMO（小泛素相关修饰剂）E3连接酶和Zip1是一个结合蛋白的SUMO共轭产物。我们的研究结果还表明，在减数分裂前期早期，Zip1与Zip3独立的Smt3缀合物相互作用（例如，Top2）促进非同源着丝粒偶联。在中前期和中前期之后，Zip1蛋白开始与Zip3依赖性Smt3缀合物缔合（例如，Red 1）在野生型细胞中沿着减数分裂染色体形成SC，在ziP3突变体中与Smt3聚合链形成PC。
The synaptonemal complex (SC) is a proteinaceous complex that apparently mediates synapsis between homologous chromosomes during meiotic prophase. In Saccharomyces cerevisiae, the Zip1 protein is the integral component of the SC. In the absence of a DNA double-strand break or the SC initiation protein Zip3, Zip1 proteins aggregate to form a polycomplex (PC). In addition, Zip1 is also responsible for DSB-independent nonhomologous centromere coupling at early meiotic prophase. We report here that Zip3 is a SUMO (small ubiquitin-related modifier) E3 ligase and that Zip1 is a binding protein for SUMO-conjugated products. Our results also suggest that at early meiotic prophase, Zip1 interacts with Zip3-independent Smt3 conjugates (e.g., Top2) to promote nonhomologous centromere coupling. At and after mid-prophase, the Zip1 protein begins to associate with Zip3-dependent Smt3 conjugates (e.g., Red1) along meiotic chromosomes in the wild-type cell to form SCs and with Smt3 polymeric chains in the ziP3 mutant to form PCs.