Molecular analysis of Klebsiella pneumoniae strains isolated in pediatric wards by ribotyping, pulsed field gel electrophoresis and antimicrobial susceptibilities.
Molecular analysis of Klebsiella pneumoniae strains isolated in pediatric wards by ribotyping, pulsed field gel electrophoresis and antimicrobial susceptibilities.
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通过核糖分型、脉冲场凝胶电泳和抗菌敏感性对儿科病房分离的肺炎克雷伯菌菌株进行分子分析。
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
G. Fadda
中科院分区：
文献类型：
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作者：
L. Sechi;T. Spanu;M. Sanguinetti;I. Dupré;L. Masucci;A. Siddu;G. Tortorolo;G. Vento;L. Maggio;A. Cambieri;S. Zanetti;G. Fadda
本研究的目的是调查不同分子分型技术在监测和控制罗马天主教大学“Agostino Gemelli”医院儿科的广谱β -内酰胺酶（ESBL）产生肺炎克雷伯菌传播中的作用，为期9个月。以HindIII为限制性内切酶，以XbaI为内切酶，采用脉冲场凝胶电泳（PFGE）对菌株进行了鉴定。在此期间分离出66株肺炎克雷伯菌临床菌株，1998年夏季分离出首批32株。在首批分离物中，核糖分型产生了26种不同的模式，而PFGE产生了16种模式。其余34株在1999年1 ~ 4月分离得到，均为ESBL生产者。核糖分型将菌株聚集成6种模式，而PFGE只产生3种模式。PCR结果显示，10株菌株同时携带bla（TEM）和bla（SHV）基因，24株菌株仅携带bla（SHV）基因。根据我们的经验，核糖分型显示出与PFGE相关的更高的分化能力，并对感染的监测有很大帮助。
The purpose of this study was to investigate the usefulness of different molecular typing techniques in the surveillance and control of the spread of extended-spectrum-beta-lactamase-(ESBL) producing Klebsiella pneumoniae in the pediatric department of the "Agostino Gemelli" hospital of the Catholic University in Rome, over a period of nine months. The strains were characterized by ribotyping using HindIII as restriction enzyme and pulsed field gel electrophoresis (PFGE) using XbaI as endonuclease. Sixty six K. pneumoniae clinical strains were isolated during this period, the first 32 were isolated in the summer of 1998. Among these first isolates, ribotyping generated 26 different patterns whereas PFGE produced 16 patterns. The remaining 34 strains were isolated during January and April 1999 and all of them were ESBL producers. Ribotyping clustered the strains into 6 patterns whereas PFGE generated only 3 patterns. PCR revealed the presence in 10 isolates of both bla(TEM) and bla(SHV) genes and 24 strains carried only the bla(SHV) gene. In our experience ribotyping revealed a higher power of differentiation with respect to PFGE and was of great help in the surveillance of the infection.