Obesity is associated with altered gene expression in human tastebuds
Obesity is associated with altered gene expression in human tastebuds
复制标题
DOI：
10.1038/s41366-018-0303-y
复制
发表时间：
2019-07-01
影响因子：
4.9
通讯作者：
Duesing, Konsta
中科院分区：
文献类型：
--
作者：
Archer, Nicholas;Shaw, Jan;Duesing, Konsta
由于心理物理和其他研究的结果相互矛盾，目前尚不清楚味觉在肥胖的发展和持续中的作用。迄今为止，还没有研究评估过瘦人和肥胖人在味觉组织的生理机能上是否存在潜在的根本差异。方法/研究对象我们分析了从瘦弱（n = 23）和肥胖（n = 13）高加索女性（年龄在18-55岁之间）活检的人真菌状味觉乳头的转录组学图谱（RNA-seq），以确定基因表达的差异。结果肥胖状况是导致个体间基因表达差异的主要因素。共有62个基因的表达量在肥胖组和瘦组之间存在显著差异（P < 0.0002），其中特异性味觉相关基因磷脂酶C β 2 （PLC β 2）和音对冲猪（SHH）的表达量在肥胖组显著降低。与炎症和免疫反应相关的基因是瘦组和肥胖组之间最丰富的生物途径。对具有双重表达变化的更广泛的基因集（2619个基因）的分析确定了三个富集的主题组（感觉知觉、细胞和突触信号传导以及免疫反应）。此外，对味觉相关基因的分析发现，与瘦子组相比，肥胖者的味觉相关基因（尤其是II型味觉细胞基因）的表达持续减少。结论肥胖与味蕾基因表达改变有关。此外，研究结果表明，瘦人与肥胖人的味蕾微环境存在明显差异，感觉基因表达的变化导致了这种微环境的改变。这项研究为肥胖和味觉改变之间的联系提供了新的证据，未来可能有助于设计对抗肥胖的策略。
Background The role of taste perception in the development and persistence of obesity is currently unclear due to conflicting results from psychophysical and other studies. No study to date has assessed whether there is an underlying fundamental difference in the physiology of taste tissue between lean and obese individuals.Method/subjects We analysed the transcriptomic profile (RNA-seq) of human fungiform taste papillae biopsied from lean (n = 23) and obese (n = 13) Caucasian females (age range 18-55) to identify differences in gene expression.Results Obesity status was the major contributor to variance in global gene expression between individuals. A total of 62 genes had significantly different gene expression levels between lean and obese (P < 0.0002), with the specific taste associated genes phospholipase C beta 2 (PLC beta 2) and sonic hedge-hog (SHH) having significantly reduced expression in obese group. Genes associated with inflammation and immune response were the top enriched biological pathways differing between the lean and the obese groups. Analysis of a broader gene set having a twofold change in expression (2619 genes) identified three enriched theme groups (sensory perception, cell and synaptic signalling, and immune response). Further, analysis of taste associated genes identified a consistent reduction in the expression of taste-related genes (in particular reduced type II taste cell genes) in the obese compared to the lean group.Conclusion The findings show obesity is associated with altered gene expression in tastebuds. Furthermore, the results suggest the tastebud microenvironment is distinctly different between lean and obese persons and, that changes in sensory gene expression contribute to this altered microenvironment. This research provides new evidence of a link between obesity and altered taste and in the future may help design strategies to combat obesity.