Effects of treadmill training combined with serotonergic interventions on spasticity after contusive spinal cord injury
Effects of treadmill training combined with serotonergic interventions on spasticity after contusive spinal cord injury
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跑步机训练联合血清素干预对脊髓挫伤后痉挛的影响
DOI：
10.1089/neu.2017.5400
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Fujita N
中科院分区：
文献类型：
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作者：
Ryu Y;Ogata T;Nishimura R. Sawada Y;Nagao M;Fujita N
痉挛状态通常出现在脊髓损伤（SCI）的恢复过程中。虽然处方药物和身体康复以减轻痉挛症状，但其效果不足仍然是一个需要解决的重要问题。考虑到增加药物剂量带来的挑战，我们假设药物和物理康复的联合治疗可能是有效的。因此，我们使用挫伤后痉挛大鼠模型检查了跑步机训练（TMT）沿着血清素药物的影响。脊髓损伤后4周，选择痉挛型SCI大鼠，分别给予以下干预2周：单纯TMT、TMT+氟西汀、TMT+赛庚啶、TMT+氟西汀、TMT+赛庚啶。我们进行了游泳试验，以量化痉挛行为的频率。我们还每周评估后肢运动功能。在干预结束时，我们检测了足底肌的霍夫曼反射和脊髓组织中5-HT 2A受体的免疫反应性。虽然TMT组和赛庚啶治疗组表现出减少痉挛行为和脊髓反射亢进，氟西汀治疗组表现出相反的效果，即使与TMT。此外，TMT抑制5-HT 2A受体在腰脊髓运动神经元的表达，而赛庚啶治疗没有改变它。我们没有观察到任何组之间的运动功能的差异。总的来说，我们的研究结果表明，TMT和赛庚啶显着缓解痉挛症状，但没有显示出协同或累加效应。
Spasticity usually emerges during the course of recovery from spinal cord injury (SCI). While medications and physical rehabilitation are prescribed to alleviate spastic symptoms, the insufficiency of their effects remains an important problem to be addressed. Given the challenges associated with increasing the dose of medication, we hypothesized that a combination therapy with medication and physical rehabilitation can be effective. Therefore, we examined the effects of treadmill training (TMT) along with serotonergic medication using a spastic rat model after contusive injury. Spasticity-strong SCI rats were selected 4 weeks after SCI and received one of the following interventions for 2 weeks: only TMT, TMT with fluoxetine (a selective serotonin re-uptake inhibitor), TMT with cyproheptadine (a 5-HT2receptor antagonist), only fluoxetine, or only cyproheptadine. We performed the swimming test to quantify the frequency of spastic behaviors. We also evaluated hindlimb locomotor functions every week. At the end of the intervention, we examined the Hoffman reflex from the plantar muscle and the immunoreactivity of the 5-HT2Areceptor in spinal cord tissues. While the TMT group and cyproheptadine-treated groups showed decreased spastic behaviors and reduction in spinal hyperreflexia, the fluoxetine-treated group showed the opposite effect, even with TMT. Moreover, TMT suppressed the expression of the 5-HT2Areceptor in the lumbar spinal motor neurons, while cyproheptadine treatment did not change it. We did not observe any differences in locomotor functions between the groups. Taken together, our findings indicate that TMT and cyproheptadine significantly alleviated spastic symptoms, but did not show synergistic or additive effects.