Local differences in immunocompetence reflect resistance of sticklebacks against the eye fluke Diplostomum pseudospathaceum
Local differences in immunocompetence reflect resistance of sticklebacks against the eye fluke Diplostomum pseudospathaceum
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DOI：
10.1017/s0031182005008681
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发表时间：
2006-01-01
期刊：
影响因子：
2.4
通讯作者：
Kurtz, J
中科院分区：
文献类型：
--
作者：
Kalbe, M;Kurtz, J
我们研究了三刺棘鱼（Gasterosteus aculeatus）对其最丰富的大型寄生虫之一——眼吸虫 Diplostomum fakespathaceum 的免疫适应的群体差异。我们比较了德国北部实验室饲养的两个鱼群的感染成功率，这两个鱼群分别来自眼吸虫盛行的湖泊和不存在这些寄生虫的河流。为了区分先天免疫和后天免疫的保护，我们只暴露鱼一次或多次。在单次接触后，湖鱼的易感性明显低于河流刺鱼，这表明在同域宿主中，先天免疫在防御这种蠕虫感染中发挥着主要作用。在这两种栖息地类型中，之前的暴露在 12 周内仅略微降低了感染率。无论感染状态如何，湖鱼通过呼吸爆发活动和脾脏大小表现出较高的免疫功能。此外，它们的能量状态比河鱼更好，肝体指数和血细胞比容值较高，表明它们的能量状态更好。有趣的是，两个种群的 F-1 杂交鱼在寄生虫易感性以及免疫和条件参数方面均介于纯栖息地类型之间。我们的结果表明，湖泊中的刺鱼更能适应该栖息地较高的寄生虫丰度。
We investigated population differences in immunological adaptation of three-spined sticklebacks (Gasterosteus aculeatus) to one of their most abundant macroparasites, the eye fluke Diplostomum pseudospathaceum. We compared infection success in lab-bred fish of 2 populations in northern Germany, from a lake, where eye flukes are prevalent, and a river, where these parasites do not occur. In order to discriminate between protection through innate and acquired immunity, we exposed fish either only once or repeatedly. Lake fish were significantly less susceptible than river sticklebacks already after a single exposure, indicating that in sympatric hosts innate immunity plays the major role in the defence against this helminth infection. In both habitat types, previous exposures only marginally decreased infection rates within 12 weeks. Lake fish showed higher immunocompentence by means of respiratory burst activity and spleen size, regardless of the infection status. Furthermore, they were in a better energy status than river fish, as indicated by a higher hepatosomatic index and haematocrit value. Interestingly, F-1 hybrid fish of both populations ranged between the pure habitat types in parasite susceptibility as well as in immunological and condition parameters. Our results suggest that sticklebacks from lakes are better adapted to cope with higher parasite abundance in this habitat.