Influence of hormone environment and donor age on cryopreserved common wombat (Vombatus ursinus) ovarian tissue xenografted into nude mice
Influence of hormone environment and donor age on cryopreserved common wombat (Vombatus ursinus) ovarian tissue xenografted into nude mice
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DOI：
10.1071/rd04054
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发表时间：
2004-01-01
影响因子：
1.9
通讯作者：
Trounson, AO
中科院分区：
文献类型：
--
作者：
Cleary, M;Shaw, JM;Trounson, AO
具有发育能力的卵母细胞可以从异种移植的卵巢组织中收集；然而，需要建立最佳的异种移植条件，使该技术用于辅助生殖。本研究将普通袋熊卵巢组织移植到裸鼠肾囊下，以阐明受体性腺状态和供体组织年龄对移植物建立、卵泡发育和卵母细胞恢复的作用。89%的移植物恢复；其中78%含有生长中的卵泡。在女性受者中，与完整受者相比，卵巢切除受者的卵泡发育到心房期的时间更早。同样，与亚成年异种卵巢组织移植相比，小袋卵巢组织移植的受者卵泡发育更早。在雄性受体小鼠肾包膜下的亚成体移植物中，卵泡发育进入心房期，尽管速度比置于雌性受体小鼠肾包膜下的亚成体移植物慢。从女性和男性受体的移植物中收集卵母细胞，但在收集时未观察到成熟的卵母细胞，这些卵母细胞也不能在体外成熟。本研究表明，将普通袋熊育儿袋幼体组织异种移植到雌性受体小鼠和将亚成年卵巢组织异种移植到雄性受体小鼠，可以发育到心房期，因此可以促进卵母细胞的收集。然而，使用目前的方法无法获得成熟的卵母细胞。
Developmentally competent oocytes can be collected from xenografted ovarian tissues; however, optimal xenograft conditions need to be established for this technique to be of use in assisted reproduction. In the present study, common wombat ovarian tissue was xenografted under the kidney capsule of nude mice to clarify the role of recipient gonadal status and donor tissue age on graft establishment, follicle development and oocyte recovery. Eighty-nine per cent of all grafts were recovered; of these, 78% contained growing follicles. In female graft recipients, follicle development to the antral stage occurred earlier in ovariectomised recipients compared with intact graft recipients. Similarly, follicle development occurred earlier in recipients of pouch young ovarian tissue grafts when compared with subadult xenografts. Follicle development proceeded to the antral stage in subadult grafts placed under the kidney capsule of male recipient mice, albeit at a slower rate than subadult grafts placed in female recipients. Oocytes were collected from grafts placed in female and male recipients, but no mature oocytes were observed at the time of collection, nor could these oocytes be matured in vitro. The present study demonstrated that common wombat pouch young tissue xenografted to female recipient mice, and subadult ovarian tissue xenografted to male recipient mice, can develop to the antral stage and can therefore facilitate oocyte collection. However, mature oocytes were not obtained using the current protocol.