The effects of ultra low fluence rate single and repetitive photodynamic therapy on glioma spheroids.
The effects of ultra low fluence rate single and repetitive photodynamic therapy on glioma spheroids.
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超低通量率单一和重复的光动力疗法对胶质瘤球体的影响。
DOI：
10.1002/lsm.20808
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发表时间：
2009-10
影响因子：
2.4
通讯作者：
Madsen, Steen J.
中科院分区：
文献类型：
--
作者：
Mathews, Marlon S.;Angell-Petersen, Even;Sanchez, Rogelio;Sun, Chung-Ho;Vo, Van;Hirschberg, Henry;Madsen, Steen J.
关键词：
通过光动力疗法 (PDT) 实现神经胶质瘤的局部控制需要将足够的光通量输送到切除边缘 1-2 厘米的深度，这是大多数局部复发的根源。由于提供足够能量所需的时间很长，这对于当前的单次术中 PDT 治疗在临床上是不切实际的。因此，似乎需要多种或延长的治疗方案。在低注量率和超低注量率下研究了使用单次或重复光传输方案的人神经胶质瘤球体对 5-氨基乙酰丙酸 (ALA) 介导的 PDT 的反应。人胶质瘤球体（直径 400 μm）接受亚阈值光通量（1.5、3 或 6 J cm−2）ALA-PDT，由四种光传递方案组成：在 1 或 24 小时内进行单次治疗，以 4 次 1 小时光治疗（间隔 4 天）进行重复治疗，或 24 小时光传递（包括 4 次 24 小时治疗，间隔 3 天）。使用生长测定评估治疗效果。在某些情况下，共聚焦显微镜用于对细胞活力进行成像。当以超低（μW cm−2）通量率进行时，重复和单次光治疗方案最为有效。在所有情况下，生长抑制均呈轻度剂量依赖性。重复超低注量率治疗（1.5 J cm−2；辐照度 = 17 μW cm−2）光传输方案是最有效的，在 2 周的观察期内导致总体生长抑制。超低光通量率 ALA-PDT 会导致显着的球体生长抑制。在重复和/或延长的单次 PDT 治疗方案中需要重复施用 ALA。这些研究中没有发现存在较低的注量率限制，低于该限制阈值光注量的功效会减弱。
Achieving local control of gliomas with photodynamic therapy (PDT) requires the delivery of adequate light fluences to depths of 1–2 cm in the resection margin where the majority of local recurrences originate. This is clinically impractical with current single-shot, intraoperative PDT treatments due to the length of time required to deliver adequate fluences. Multiple or extended treatment protocols would therefore seem to be required. The response of human glioma spheroids to 5-aminolevulinic acid (ALA)-mediated PDT using single or, repetitive light delivery protocols was investigated at both low and ultra low fluence rates. Human glioma spheroids (400 μm diameter) were subjected to sub-threshold light fluence (1.5, 3, or 6 J cm−2) ALA–PDT consisting of four light delivery schemes: single treatment given over either 1 or 24 hours, repetitive treatment given either as four 1 hour light treatments separated by a 4 day interval, or 24 hours light delivery, consisting of four 24 hours treatments separated by a 3 day interval. Treatment efficacy was evaluated using a growth assay. In some cases, confocal microscopy was used to image cell viability. The repetitive and single light treatment protocols were most effective when delivered at ultra low (μW cm−2) fluence rates. In all cases, growth inhibition was light dose-dependent. The repetitive ultra low fluence rate treatment (1.5 J cm−2; irradiance = 17 μW cm−2) light delivery protocol was the most effective resulting in total growth inhibition during the 2-week observation period. Ultra low light fluence rate ALA–PDT results in significant spheroid growth inhibition. Repeated administration of ALA was required during repetitive and/or protracted single PDT treatment protocols. The existence of a lower fluence rate limit, below which the efficacy of threshold light fluences diminish was not found in these studies.
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影响因子：
2.4
作者：
Hirschberg, Henry;Uzal, Francisco A.;Chighvinadze, David;Zhang, Michelle J.;Peng, Qian;Madsen, Steen J.
通讯作者：
Madsen, Steen J.
DOI：
10.1089/clm.1998.16.81
发表时间：
1998-04-01
期刊：
JOURNAL OF CLINICAL LASER MEDICINE & SURGERY
影响因子：
--
作者：
Lilge, L;Wilson, BC
通讯作者：
Wilson, BC
DOI：
10.1016/1011-1344(90)85083-9
发表时间：
1990-06-01
影响因子：
5.4
作者：
KENNEDY, JC;POTTIER, RH;PROSS, DC
通讯作者：
PROSS, DC
影响因子：
3.3
作者：
Bisland, SK;Lilge, L;Wilson, BC
通讯作者：
Wilson, BC
影响因子：
3.1
作者：
Bogaards, A;Varma, A;Wilson, BC
通讯作者：
Wilson, BC