Gut microbiota and diet in patients with different glucose tolerance.
Gut microbiota and diet in patients with different glucose tolerance.
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DOI：
10.1530/ec-15-0094
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发表时间：
2016-01
影响因子：
2.9
通讯作者：
Boytsov S
中科院分区：
文献类型：
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作者：
Egshatyan L;Kashtanova D;Popenko A;Tkacheva O;Tyakht A;Alexeev D;Karamnova N;Kostryukova E;Babenko V;Vakhitova M;Boytsov S
2型糖尿病（T2 D）是一种严重的疾病。肠道微生物群（GM）最近被确定为除了众所周知的糖尿病风险因素之外的新的潜在风险因素。为了研究不同糖耐量患者的GM组成与饮食模式的关系，我们分析了92例患者：正常糖耐量（n=48），前驱糖尿病（preD，n=24）和T2 D（n=20）。使用16 S rRNA测序进行宏基因组学分析。通过频率法研究了饮食，并使用计算机程序对食物摄入量进行了定量评价。样品中的微生物群主要由厚壁菌门（Firmicutes）代表，其次为拟杆菌门（Bacteroidetes）。布劳特氏菌属是所有样品中的优势属。布劳特氏菌属、沙雷氏菌属在preD中的代表性低于T2 D患者，在糖耐量正常的患者中甚至更低。根据蛋白质、脂肪、碳水化合物的含量对样品进行聚类后，类杆菌在碳水化合物中的代表性降低，而普雷沃菌在碳水化合物中的丰度升高。在脂肪-蛋白质组中，胰岛素抵抗、T2 D患者较多。使用Calinski-Harabasz指数确定了具有更相似饮食的样本。结果发现，高脂肪饮食的患者中有一半具有正常的耐受性，其他人患有T2 D。回归分析显示，这些T2 D患者的布劳特氏菌感染率也较高。我们的研究提供了进一步的证据，关于结构调节的GM在2型糖尿病发病机制取决于饮食模式。
Type 2 diabetes (T2D) is a serious disease. The gut microbiota (GM) has recently been identified as a new potential risk factor in addition to well-known diabetes risk factors. To investigate the GM composition in association with the dietary patterns in patients with different glucose tolerance, we analyzed 92 patients: with normal glucose tolerance (n=48), prediabetes (preD, n=24), and T2D (n=20). Metagenomic analysis was performed using 16S rRNA sequencing. The diet has been studied by a frequency method with a quantitative evaluation of food intake using a computer program. Microbiota in the samples was predominantly represented by Firmicutes, in a less degree by Bacteroidetes. Blautia was a dominant genus in all samples. The representation of Blautia, Serratia was lower in preD than in T2D patients, and even lower in those with normal glucose tolerance. After the clustering of the samples into groups according to the percentage of protein, fat, carbohydrates in the diet, the representation of the Bacteroides turned to be lower and Prevotella abundance turned to be higher in carbohydrate cluster. There were more patients with insulin resistance, T2D in the fat–protein cluster. Using the Calinski–Harabasz index identified the samples with more similar diets. It was discovered that half of the patients with a high-fat diet had normal tolerance, the others had T2D. The regression analysis showed that these T2D patients also had a higher representation of Blautia. Our study provides the further evidence concerning the structural modulation of the GM in the T2DM pathogenesis depending on the dietary patterns.