A compact light-sheet microscope for the study of the mammalian central nervous system.
A compact light-sheet microscope for the study of the mammalian central nervous system.
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DOI：
10.1038/srep26317
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发表时间：
2016-05-24
影响因子：
4.6
通讯作者：
Dholakia K
中科院分区：
文献类型：
--
作者：
Yang Z;Haslehurst P;Scott S;Emptage N;Dholakia K
对神经元功能不可或缺的瞬态过程的研究需要大视场的快速、高分辨率成像技术，这是传统扫描显微镜无法实现的。在这里，我们描述了一款紧凑型光片荧光显微镜，具有 45° 倒置几何结构和集成光解激光器，针对神经科学应用进行了优化，特别是哺乳动物脑切片中亚神经元结构的快速成像。我们展示了这种设计在三维形态重建、局部光解激活单个突触以及大视场神经元 Ca2+ 信号传导快速成像方面的实用性。开发的系统为神经科学界开辟了许多新颖的应用。
Investigation of the transient processes integral to neuronal function demands rapid and high-resolution imaging techniques over a large field of view, which cannot be achieved with conventional scanning microscopes. Here we describe a compact light sheet fluorescence microscope, featuring a 45° inverted geometry and an integrated photolysis laser, that is optimized for applications in neuroscience, in particular fast imaging of sub-neuronal structures in mammalian brain slices. We demonstrate the utility of this design for three-dimensional morphological reconstruction, activation of a single synapse with localized photolysis, and fast imaging of neuronal Ca2+ signalling across a large field of view. The developed system opens up a host of novel applications for the neuroscience community.