Genetic analysis of group B human rotaviruses detected in Bangladesh in 2000 and 2001
Genetic analysis of group B human rotaviruses detected in Bangladesh in 2000 and 2001
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DOI：
10.1002/jmv.10546
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发表时间：
2004-01-01
影响因子：
12.7
通讯作者：
Sumi, A
中科院分区：
文献类型：
--
作者：
Ahmed, MU;Kobayashi, N;Sumi, A
对2000年和2001年在孟加拉国检测到的B组轮状病毒进行了遗传分析，以澄清与中国和印度先前报告的人类B组轮状病毒以及动物B组轮状病毒的相关性。孟加拉国B组轮状病毒（Bang 373、Bang 544、Bang 334和Bang 402）的VP 7基因序列彼此几乎相同，并且与印度株CAL-1（98%）和中国株成人腹泻轮状病毒（ADRV）（92%）也显示出高序列同一性，而与牛和鼠病毒的同一性相当低（60-63%）。Bang 373和Bang 544编码与NSP 5相似的VP 2、VP 4、VP 6和NSP 1的基因与CAL-1的同源性（97.7-99.4%）远高于ADRV的同源性（89.9-93.9%）。Bang 373、CAL-1和ADRV之间核苷酸取代的表征表明，所有基因片段可能以相似的突变率中性进化，而一些基因片段（例如，VP 2基因）的保守性高于其它基因。总之，在孟加拉国检测到的B组轮状病毒（以Bang 373和印度病毒CAL-1为代表）被认为是与ADRV在遗传上不同的几乎相同的病毒，并表明Bang 373（CAL-1）样B组轮状病毒（孟加拉毒株）可能主要分布在孟加拉湾周围地区。
Group B rotaviruses detected in Bangladesh in 2000 and 2001 were analyzed genetically to clarify relatedness to human group B rotaviruses reported previously in China and India, and to animal group B rotaviruses. VP7 gene sequences of the Bangladeshi group B rotaviruses (Bang373, Bang544, Bang334, and Bang402) were almost identical to each other and also showed high sequence identity to the Indian strain CAL-1 (98%) and Chinese strain adult diarrhea rotavirus (ADRV) (92%), while identities to bovine and murine viruses were considerably low (60-63%). Other genes of Bang373 and Bang544 encoding VP2, VP4, VP6, and NSP1 similar to NSP5 also showed much higher sequence identities to those of CAL-1 (97.7-99.4%) than to those of ADRV (89.9-93.9%). Characterization of nucleotide substitutions among Bang373, CAL-1, and ADRV suggested that all the gene segments might have evolved neutrally at similar mutation rates, while some of the gene segments (e.g., VP2 gene) were suggested to be more conserved than others. In conclusion, group B rotaviruses detected in Bangladesh represented by Bang373 and the Indian virus CAL-1 were considered as virtually identical viruses which are distinct genetically from ADRV, and it was suggested that Bang373 (CAL-1)-like group B rotavirus (Bengali strains) might be distributed primarily in an area around the Bay of Bengal.