Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae
Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae
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DOI：
10.1016/s0378-1097(99)00027-0
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发表时间：
1999-03-15
影响因子：
2.1
通讯作者：
Camilli, A
中科院分区：
文献类型：
--
作者：
Bricker, AL;Camilli, A
肺炎链球菌菌株JNR.7/87是一种高毒力的4型包封革兰氏阳性细菌，其可转化性先前尚未测试，并且其基因组目前正在测序。通过添加外源性感受态刺激肽以非常低的效率转化该菌株：然而，该效率太低且不可重现，以致于在许多遗传研究中是有用的。因此，已经检查了改变感受态刺激肽诱导的转化的不同组分对转化效率的影响。筛选生长培养基，然后优化预诱导培养酸化，甘氨酸浓度和诱导时间。建立了S.用链霉素抗性（Sm-R）标记以类似于每10（8）个细胞10（5）个菌落形成单位的效率可重复地转化肺炎杆菌菌株JNR.7/87。(C)1999年，欧洲微生物学会联合会。由Elsevier Science B. V.出版，版权所有。
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (Sm-R) marker at an efficiency of similar to 10(5) colony forming units per 10(8) cells. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.