Radiation-induced γ-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam
Radiation-induced γ-H2AX in mammalian cells irradiated with a synchrotron X-ray microbeam
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DOI：
10.1093/rpd/ncl434
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发表时间：
2006-12-01
影响因子：
1
通讯作者：
Kobayashi, K.
中科院分区：
文献类型：
--
作者：
Usami, N.;Maeda, M.;Kobayashi, K.
为了研究低剂量辐射的放射生物学效应，研制了一种利用同步加速器X射线微束照射细胞的系统，该系统可以对单个细胞进行识别，并用所需剂量的单色X射线逐个照射细胞。使用聚焦光学器件获得的最小光束尺寸为2 μ m，使用非聚焦光学器件获得的最小光束尺寸为5 μ m²，并且在非聚焦光学器件的情况下，可以通过高精度狭缝轻松改变光束尺寸。用该系统单独照射人成纤维细胞，并用γ - h2ax抗体进行免疫染色，以显示DNA损伤。大多数荧光灶在细胞核的局部区域观察到，其大小与光束大小几乎相同。
In order to study the radiobiological effects from low dose radiation, a cell irradiation system using synchrotron X-ray microbeam has been developed, by which cells can be recognised individually and irradiated one by one with the desired dose of monochromatic X rays. The minimum beam sizes obtained are 2 mu m with the focusing optics and 5 mu m square with the non-focused beam, and the beam size can be changed easily with a high-precision slit in the case of a non-focused beam. Human fibroblast cells were individually irradiated with this system, and immunostained by gamma-H2AX antibody to visualise the DNA damage. Most of the fluorescent foci were observed in a localised area in cell nuclei, the size of which was almost the same as the beam size.