1-ALPHA,25-DIHYDROXYVITAMIN D-3 INHIBITS THE INVASIVE POTENTIAL OF HUMAN BREAST-CANCER CELLS IN-VITRO
1-ALPHA,25-DIHYDROXYVITAMIN D-3 INHIBITS THE INVASIVE POTENTIAL OF HUMAN BREAST-CANCER CELLS IN-VITRO
复制标题
DOI：
10.1007/bf01753887
复制
发表时间：
1994-05-01
影响因子：
4
通讯作者：
BINDERUP, L
中科院分区：
文献类型：
--
作者：
HANSEN, CM;FRANDSEN, TL;BINDERUP, L
使用Boyden小室侵袭试验，检查1 α，25-二羟基维生素D-3 [1 α，25（OH）（2）D-3]对高度侵袭性雌激素受体阴性人乳腺癌细胞系MDA-MB-231的侵袭性的影响。MDA-MB-231细胞显示含有1 α，25（OH）（2）D-3的高亲和力受体，Kd为1.5 × 10（-11）M。当在进行测定之前用1 α，25（OH）（2）D-3处理细胞4天时，证明了其侵袭潜力的剂量依赖性抑制。浓度为13 pM的1 α，25（OH）（2）D-3可抑制50%的侵袭。然而，当细胞在测定期间仅处理6小时时，未观察到抑制作用。迁移过程也受到1 α，25（OH）（2）D-3处理4天的影响，尽管抑制程度与侵袭不同。浓度为3.2 nM的1 α，25（OH）（2）D-3（比侵袭试验高250倍）对迁移的抑制率为50%。侵袭和迁移的抑制不是由于1 α，25（OH）（2）D-3的已知抗增殖作用，因为在长达5天的处理中未证明生长减少。因此，根据目前的研究，可以得出结论，1 α，25（OH）（2）D-3能够通过一种机制抑制肿瘤细胞的侵袭性，该机制不仅仅基于其抗增殖和抗迁移作用。
Using the Boyden chamber invasion assay, the effect of 1 alpha,25-dihydroxyvitamin D-3 [1 alpha,25(OH)(2)D-3] on the invasiveness of the highly invasive, oestrogen receptor-negative human breast cancer cell line MDA-MB-231 was examined. The MDA-MB-231 cells were shown to contain high-affinity receptors for 1 alpha,25(OH)(2)D-3 with a K-d of 1.5 X 10(-11) M. When the cells were treated with 1 alpha,25(OH)(2)D-3 for 4 days before the assay was performed, a dose-dependent inhibition of their invasive potential was demonstrated. Fifty per cent inhibition of invasion was obtained with a concentration of 13 pM of 1 alpha,25(OH)(2)D-3. However, when the cells were treated for only 6 h during the assay, no inhibitory effect was seen. The process of migration was also affected by treatment with 1 alpha,25(OH)(2)D-3 for 4 days, although the inhibition was not of the same magnitude as seen for the invasion. Fifty per cent inhibition of migration occurred at a concentration of 3.2 nM of 1 alpha,25(OH)(2)D-3 (250 times higher than in the invasion assay). Inhibition of invasion and migration was not due to the known anti-proliferative effect of 1 alpha,25(OH)(2)D-3, as no growth reduction could be demonstrated with treatment up to 5 days. Based on the present investigation it can therefore be concluded that 1 alpha,25(OH)(2)D-3 is able to inhibit tumour cell invasiveness by a mechanism which is not exclusively based on its anti-proliferative and anti-migrative effects.