Are all leucodepleted platelet concentrates equivalent? Comparison of Cobe LRS Turbo, haemonetics MCS plus LD, and filtered pooled buffy-coat-derived platelets
Are all leucodepleted platelet concentrates equivalent? Comparison of Cobe LRS Turbo, haemonetics MCS plus LD, and filtered pooled buffy-coat-derived platelets
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DOI：
10.1159/000031176
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发表时间：
2000-01-01
期刊：
影响因子：
2.7
通讯作者：
Seghatchian, J
中科院分区：
文献类型：
--
作者：
Krailadsiri, P;Seghatchian, J
背景和目的：有许多技术可用于生产去白细胞血小板浓缩物（PC）。本研究比较了三种常用技术制备的PC的特性。材料与方法：将通过Cobe LRS Turbo单采系统、Haemonetics MCS+（配备在线过滤器）和来自合并血沉棕黄层（BC）的PC过滤制备的总计15个单位的去白细胞PC转移至相同的标准容器中。测量用于评估血小板的状态/活化和微泡形成以及血小板损伤的标志物。结果如下：在所有类型的PC中，pH均保持良好。第1天，Cobe LRS Turbo中CD 62 P的表达较高，但在第5天，三种方法之间的表达相等。血小板表面CD 62 P表达与血浆可溶性CD 62 P表达呈显著相关。第1天，Cobe LRS Turbo和BC-PC中的磷脂酰丝氨酸（PS）暴露程度略高于Haemonetics MCS+。然而，在第5天，两种单采术PC值均高于BC-PC。PS暴露与微泡形成之间存在显著相关性。结论：3种方法制备的去白细胞PC在贮存损伤和微泡形成方面存在差异。这些发现的临床意义仍有待研究。版权所有（C）2000 S. Karger AG，巴塞尔。
Background and Objectives: A number of technologies are available for the production of leucocyte-depleted platelet concentrates (PCs). This study compared the characteristics of PCs prepared by three commonly used techniques. Materials and Methods: In total, fifteen units of leucoyte-depleted PCs prepared by the Cobe LRS Turbo apheresis system, Haemonetics MCS+ with in-line filter and filtration of PCs derived from pooled buffy coats (BCs) were transferred into the same standard container. Markers to assess status/activation and microvesiculation of platelets as well as platelet injury were measured. Results: pH was well maintained in all types of PCs, The expression of CD62P was higher in Cobe LRS Turbo on day 1 but became equivalent between the three methods on day 5. A significant correlation was found between the expression of CD62P on platelet surface and soluble CD62P in the plasma. The degree of phosphatidylserine (PS) exposure was slightly higher in Cobe LRS Turbo and BC-PCs than Haemonetics MCS+ on day 1. However, on day 5 both apheresis PC values were higher than BC-PCs. A significant correlation was found between PS exposure and microvesiculation, Conclusion: Leucodepleted PCs prepared by the three methods were different in terms of storage lesion and microvesiculation. The clinical significance of these findings remains to be investigated. Copyright (C) 2000 S. Karger AG, Basel.