The Balancing Act of Ribonucleotides in DNA.
The Balancing Act of Ribonucleotides in DNA.
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DOI：
10.1016/j.tibs.2016.02.005
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发表时间：
2016-05
影响因子：
13.8
通讯作者：
Crouch RJ
中科院分区：
文献类型：
--
作者：
Cerritelli SM;Crouch RJ
DNA中核糖核苷酸的丰度直到最近才被检测到，因为它们被核糖核苷酸切除修复途径有效地去除，这一过程类似于RNA酶H2切割后的冈崎片段加工。所有的DNA聚合酶在DNA合成过程中都会掺入核糖核苷酸。有多少，何时以及为什么它们被合并一直是近年来许多实验室紧张工作的焦点。在这篇综述中，我们讨论了核糖核苷酸掺入真核生物DNA聚合酶的最新进展，这表明核糖核苷酸在DNA中的进化保守作用，并回顾了表明核糖核苷酸的去除在维持基因组稳定性方面起着重要作用的数据。
The abundance of ribonucleotides in DNA remained undetected until recently because they are efficiently removed by the Ribonucleotides Excision Repair pathway, a process similar to Okazaki fragment processing after incision by RNase H2. All DNA polymerases incorporate ribonucleotides during DNA synthesis. How many, when and why they are incorporated has been the focus of intense work during recent years by many labs. In this review, we discuss recent advances in ribonucleotide incorporation by eukaryotic DNA polymerases that suggest an evolutionarily conserved role for ribonucleotides in DNA and review the data that indicate that removal of ribonucleotides plays an important role in maintaining genome stability.