Mechanism of reduction in the number of the circulating hemocytes in the Pseudaletia separata host parasitized by Cotesia kariyai.
Mechanism of reduction in the number of the circulating hemocytes in the Pseudaletia separata host parasitized by Cotesia kariyai.
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DOI：
10.1016/j.jinsphys.2004.08.005
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发表时间：
2004-12
影响因子：
2.2
通讯作者：
Tokiyasu Teramoto;Toshiharu Tanaka
中科院分区：
文献类型：
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作者：
Tokiyasu Teramoto;Toshiharu Tanaka
寄生蜂幼虫可通过多DNA病毒（PDV）和毒液的作用逃避宿主的免疫反应。PDV感染血细胞并影响宿主的血细胞功能。在本文中，我们研究了PDV和毒液如何影响宿主的血细胞群体。幼虫的内寄生蜂卡氏盘绒茧蜂在寄生后不久就降低了寄主幼虫的血细胞数量。循环血细胞数量的减少是由循环血细胞和产生循环血细胞的造血器官的分解引起的。寄生后不久血细胞数量的减少是对毒液的反应。然而，在寄生后6小时和之后的血细胞群体的减少似乎是由PDV引起的。PDV加蛇毒注射后6h及注射后6h，循环血细胞出现凋亡。血细胞计数结果显示，PDV加蛇毒注射后24小时内循环血细胞的有丝分裂停止。注射PDV+蛇毒后12h，造血器官出现凋亡。此外，血浆从宿主注射PDV加毒液抑制血细胞从造血器官释放的数量。
Larval endoparasitoids can avoid the immune response of the host by the function of polydnavirus (PDV) and venom. PDV infects hemocytes and affects the hemocyte function of the host. In this paper, we investigated how PDV and venom affect the hemocyte population of the host. Cotesia kariyai, the larval endoparasitoid, lowers the hemocyte population of the noctuid host larvae soon after parasitization. The reduction in the number of circulating hemocytes is caused by the breakdown of the circulating hemocytes and of the hematopoietic organ which generates the circulating hemocytes. The decrease in the number of hemocytes shortly after parasitization is a response to the venom. However, the decrease in hemocyte population on and after 6h post-parasitization appears to be caused by the PDV. Apoptosis in circulating hemocytes was observed on and after 6h post-injection of PDV plus venom. It was revealed through cytometry that mitosis of circulating hemocytes was halted within 24h after the injection of PDV plus venom. Apoptosis in the hematopoietic organ was induced 12h after the injection of PDV plus venom. Furthermore, the plasma from the hosts injected with PDV plus venom depressed the number of hemocytes released from the hemotopoiteic organs.