Anti-Candida and anti-Cryptococcus antifungal produced by marine microorganisms.
Anti-Candida and anti-Cryptococcus antifungal produced by marine microorganisms.
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DOI：
10.1016/j.mycmed.2014.04.004
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发表时间：
2014-12-01
影响因子：
3.6
通讯作者：
Fassouane, A
中科院分区：
文献类型：
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作者：
El Amraoui, B;El Amraoui, M;Fassouane, A
为了寻找生物来源的抗真菌药物，我们对从摩洛哥大西洋不同沿海地区收集的海水、海藻、沉积物和海洋无脊椎动物中分离的海洋微生物进行了筛选。研究了这些分离物对医学真菌学病原酵母菌的抑菌活性。采用琼脂扩散法筛选了34种海洋微生物的全培养物对4种酵母的抑菌活性。结果表明，在34株分离菌株中，13株（38%）对4种酵母菌中至少1种具有抗真菌活性，11株具有抗白色念珠菌CIP 48.72活性，12株具有抗c活性。对制霉菌素和两性霉素b耐药的热带念珠菌R2有活性的菌株只有6株，对白色念珠菌CIP 884.65有较强的活性。鉴定出14种微生物，归属于不动杆菌属、气单胞菌属、碱性单胞菌属、芽孢杆菌属、色杆菌属、肠球菌属、泛菌属和假单胞菌属。由于空间和养分的竞争作用，海洋微生物可以产生更多的抗菌剂；因此，这些海洋微生物有望成为我们研究的抗念珠菌和抗隐球菌杀菌剂等天然产物的潜在资源。
In order to search for antifungal from biological origin, we performed a screening of marine microorganisms isolated from seawater, seaweed, sediment and marine invertebrates collected from different coastal areas of the Moroccan Atlantic Ocean. The antifungal activities of these isolates were investigated against the pathogenic yeasts involved in medical mycology. Whole cultures of 34 marine microorganisms were screened for antifungal activities using the method of agar diffusion against four yeasts. The results showed that among the 34 isolates studied, 13 (38%) strains have antifungal activity against at least one out of four yeast species, 11 isolates have anti-Candida albicans CIP 48.72 activity, 12 isolates have anti-C. albicans CIP 884.65 activity, 13 isolates have anti-Cryptococcus neoformans activity and only 6 isolates are actives against Candida tropicalis R2 resistant to nystatin and amphotericin B. Nine isolates showed strong fungicidal activity. Fourteen microorganisms were identified and assigned to the genera Acinetobacter, Aeromonas, Alcaligenes, Bacillus, Chromobacterium, Enterococcus, Pantoea, and Pseudomonas. Due to a competitive role for space and nutrient, the marine microorganisms could produce more antimicrobials; therefore these marine microorganisms were expected to be potential resources of natural products such as those we research: anti-Candida and anti-Cryptococcus fungicides.