Microwave-assisted synthesis of chitosan biguanidine hydrochloride and its antioxidant activity in vitro
Microwave-assisted synthesis of chitosan biguanidine hydrochloride and its antioxidant activity in vitro
复制标题
微波辅助合成壳聚糖盐酸双胍及其体外抗氧化活性
DOI：
10.1002/app.43570
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发表时间：
2016
影响因子：
3
通讯作者：
Wu Yuntang
中科院分区：
文献类型：
--
作者：
Liu Qianwen;Li Yuan;Jin Xin;Peng Qin;Liu Xiaofei;Wu Yuntang
在微波辐射下，以壳聚糖盐酸盐（CSH）为原料，通过胍基化反应合成了壳聚糖双胍盐酸盐（CSGH），并采用傅里叶变换红外光谱、凝胶渗透色谱和元素分析等方法对其进行了表征。采用化学法和细胞法研究了CSGH的抗氧化活性。结果表明，CSGH对2，2-二苯基-1-苦基-肼基（DPPH.）自由基的清除能力优于CSH，略逊于抗坏血酸（Vc），且CSGH对DPPH.的清除能力具有剂量依赖性。细胞实验表明，CSGH对氧化损伤的细胞具有上级修复作用。CSGH的干预可以很好地恢复细胞形态，降低丙二醛水平，提高超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。CSGH是一种潜在的抗氧化剂，其抗氧化活性不仅体现在清除自由基方面，还体现在调节受损细胞的氧化/抗氧化平衡方面。© 2016 Wiley Periodicals，Inc. J.应用聚合物Sci.2016，133，43570.
Chitosan biguanidine hydrochloride (CSGH) was synthesized by guanidinylation of chitosan hydrochloride (CSH) under microwave irradiation and characterized by Fourier transform infrared spectroscopy, gel permeation chromatography, and element analysis. The antioxidant activity of CSGH was evaluated by both chemical and cellular methods. The results showed that CSGH was better than CSH but slightly inferior to ascorbic acid (Vc) for scavenging 2,2‐diphenyl‐1‐picryl‐hydrazyl (DPPH) free radicals, and the DPPH• scavenging ability of CSGH was dose‐dependent. However, cell experiments indicated CSGH had a superior repairing effect on oxidatively damaged cells. The intervention of CSGH could excellently recover the cell morphology, decrease the level of malondialdehyde, and enhance the activity of superoxide dismutase and glutathione peroxidase. CSGH could be a potential antioxidant reagent, and its antioxidant activity was reflected not only in scavenging the free radicals but also in regulating the oxidation/antioxidation balance of damaged cells. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2016,133, 43570.