Structure of synthetic unmethylated 16S ribosomal RNA as purified RNA and in reconstituted 30S ribosomal subunits.
Structure of synthetic unmethylated 16S ribosomal RNA as purified RNA and in reconstituted 30S ribosomal subunits.
复制标题
合成的未甲基化 16S 核糖体 RNA 的结构（纯化 RNA 和重建的 30S 核糖体亚基）。
DOI：
10.1021/bi00441a043
复制
发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Wollenzien,P
中科院分区：
文献类型：
--
作者：
Ericson,G;Chevli,K;Wollenzien,P
用克隆基因体外转录的方法制备16S核糖体RNA，并与16S核糖体RNA进行结构比较。比较的方法是将这两种类型的16S rRNA置于与未配对碱基特异反应的化学试剂中，并通过逆转录和凝胶电泳法确定反应的程度。在溶液中，除了A1408处的差异和残基470和562之间的区域的许多差异外，rRNA在其反应模式上是不可区分的。当16S rRNA重组为30S核糖体亚基时，这些反应性差异就消失了。当人工合成的16S rRNA重组为30S亚基后，提取并在溶液中检测，其在470-562区的化学反应模式与真正的16S rRNA相同，但在1408-1410区的差异仍然存在。本研究表明，合成的16S rRNA在溶液中具有类似于天然二级结构的二级结构，但在两个区域，一个明显在合成过程中错误折叠，另一个可能是真正的16S rRNA中通常甲基化的核苷酸导致结构差异的原因。
16S ribosomal RNA was made by in vitro transcription of a cloned gene, and its structure was compared to authentic 16S ribosomal RNA. The comparison was made by subjecting the two types of 16S rRNA to chemical reagents that react specifically with unpaired bases and determining the extent of reaction by reverse transcription and gel electrophoresis of the cDNA. In solution, the rRNAs were indistinguishable in their pattern of reactivity, except for a difference at A1408 and many differences in the region between residues 470 and 562. When the 16S rRNAs were reconstituted into 30S ribosomal subunits, these reactivity differences were absent. When the synthetic 16S rRNA was reconstituted into 30S subunits andthen extracted and tested in solution, its pattern of chemical reactivity in the 470-562 region was the same as authentic 16S rRNA, but differences in the 1408-1410 region persisted. This study indicates that the synthetic 16S rRNA has a secondary structure in solution similar to a native secondary structure except in two regions, one apparently incorrectly folded during synthesis and the other in which nucleotides which are normally methylated in authentic 16S rRNA may be responsible for a structural difference.
登录
查看更多内容
大肠杆菌16S核糖体RNA 3端附近两个相邻N6,N6-二甲基腺苷的功能研究。
影响因子：
4.8
作者：
B. Poldermans;C. V. van Buul;P. van Knippenberg
通讯作者：
P. van Knippenberg
[47] 具有多肽合成活性的大肠杆菌核糖体亚基的制备☆
影响因子：
--
作者：
T. Staehelin;D. Maglott
通讯作者：
D. Maglott
DOI：
10.1073/pnas.85.4.1033
发表时间：
1988-02-01
影响因子：
11.1
作者：
SAMPSON, JR;UHLENBECK, OC
通讯作者：
UHLENBECK, OC
影响因子：
5.6
作者：
MOAZED, D;STERN, S;NOLLER, HF
通讯作者：
NOLLER, HF
使用热变性的 16-S rRNA 体外重建活性 30-S 核糖体亚基。
DOI：
--
发表时间：
1979
期刊：
European Journal of Biochemistry
影响因子：
--
作者：
D. Barritault;M. F. Guerin;D. Hayes
通讯作者：
D. Hayes