CDDP induces p53-dependent apoptosis in tongue cancer cells.
CDDP induces p53-dependent apoptosis in tongue cancer cells.
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CDDP 诱导舌癌细胞 p53 依赖性细胞凋亡。
DOI：
10.3892/ijo.17.3.513
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发表时间：
2000
影响因子：
5.2
通讯作者：
H. Hosoi
中科院分区：
文献类型：
--
作者：
H. Kanata;K. Yane;I. Ota;H. Miyahara;T. Matsunaga;A. Takahashi;K. Ohnishi;T. Ohnishi;H. Hosoi
我们研究了两种具有不同p53遗传状态的舌癌细胞系的CDDP敏感性，一种是野生型p53（SAS），另一种是突变型p53（HSC-4）。SAS在D10剂量下的敏感性约为2倍，并且在CDDP处理后10小时，Western印迹分析显示p53和Bax蛋白水平增加。另一方面，在没有CDDP处理的情况下观察到HSC-4中p53的过表达，并且没有检测到Bax的升高。Hoechst 33342染色和电泳法观察到CDDP处理后SAS细胞出现凋亡，而HSC-4细胞未见凋亡。这些发现表明p53作为Bax表达的正性调节因子在细胞凋亡中起重要作用。这表明p53状态可能对CDDP治疗的反应具有预测潜力。
We have investigated the CDDP sensitivities of two tongue cancer cell lines with differing p53 genetic status, one with wild-type p53 (SAS) and the other with mutant-type p53 (HSC-4). SAS was about 2 times more sensitive at the D10 dose and demonstrated increased p53 and Bax protein levels at 10 h after CDDP treatment on Western blot analysis. On the other hand, overexpression of p53 in HSC-4 was observed without CDDP treatment and no elevation of Bax could be detected. Apoptosis was observed after CDDP treatment in SAS but not in HSC-4 by Hoechst 33342-staining and electrophoresis methods. These findings indicate that p53 plays an important role in apoptosis as a positive regulator of Bax expression. It is suggested that p53 status may have predictive potential with regard to response to CDDP therapy.