Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes
Effects of TGF-β1 and IGF-I on the compressibility, biomechanics, and strain-dependent recovery behavior of single chondrocytes
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DOI：
10.1016/j.jbiomech.2007.12.006
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Koay, Eugene J.;Ofek, Gidon;Athanasiou, Kyriacos A.
关节软骨细胞对物理化学刺激的反应与可导致退行性和再生过程的过程密切相关。为了理解这种联系，我们研究了单个软骨细胞对生长因子（IGF-I和tgf - β 1）和一系列压缩应变的生物力学行为。结果表明，生长因子在细胞的刚度系数（类似于比对照增加两倍）和压缩性方面改变了细胞的生物力学，这是由明显的泊松比测量的（类似于也比对照增加两倍）。有趣的是，相对于施加的应变，压缩率显著降低。此外，我们再次在所有治疗中检测到软骨细胞的临界应变阈值，类似于30%的应变。总的来说，这些发现表明细胞生物力学在生化和生物力学扰动下都会发生变化。了解软骨细胞对这些刺激反应的潜在生物力学可能有助于理解软骨的各个方面，包括骨关节炎的研究和组织工程策略的发展。(c) 2007 Elsevier Ltd.版权所有。
The responses of articular chondrocytes to physicochemical stimuli are intimately linked to processes that can lead to both degenerative and regenerative processes. Toward understanding this link, we examined the biomechanical behavior of single chondrocytes in response to growth factors (IGF-I and TGF-beta 1) and a range of compressive strains. The results indicate that the growth factors alter the biomechanics of the cells in terms of their stiffness coefficient (similar to two-fold increase over control) and compressibility, as measured by an apparent Poisson's ratio (similar to two-fold increase over control also). Interestingly, the compressibility decreased significantly with respect to the applied strain. Moreover, we have again detected a critical strain threshold in chondrocytes at similar to 30% strain in all treatments. Overall, these findings demonstrate that cellular biomechanics change in response to both biochemical and biomechanical perturbations. Understanding the underlying biomechanics of chondrocytes in response to such stimuli may be useful in understanding various aspects of cartilage, including the study of osteoarthritis and the development of tissue-engineering strategies. (c) 2007 Elsevier Ltd. All rights reserved.