Ovarian hormone effects on 5-hydroxytryptamine2A and 5-hydroxytryptamine2C receptor mRNA expression in the ventral hippocampus and frontal cortex of female rats
Ovarian hormone effects on 5-hydroxytryptamine2A and 5-hydroxytryptamine2C receptor mRNA expression in the ventral hippocampus and frontal cortex of female rats
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DOI：
10.1016/s0304-3940(01)02570-8
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发表时间：
2002-02-22
影响因子：
2.5
通讯作者：
Olsson, T
中科院分区：
文献类型：
--
作者：
Birzniece, V;Johansson, IM;Olsson, T
女性性腺激素的改变与焦虑和情绪变化有关。本研究的目的是确定慢性性激素补充对腹侧海马和额叶大脑皮层5-HT 2A和5-HT 2C受体mRNA水平的影响。切除卵巢的成年雌性Sprague-Dawley大鼠（n = 37）接受皮下植入含有不同剂量的17 β-雌二醇单独或与孕酮组合或安慰剂颗粒的颗粒，持续2周。5-羟色胺受体mRNA水平进行了分析，在腹侧海马和5-HT 2A受体mRNA也在额叶皮质原位杂交。雌二醇与低剂量孕酮联合治疗使腹侧海马CA 2区5-HT 2A受体mRNA增加43%，而雌二醇与高剂量孕酮联合治疗使腹侧CA 1区该基因的表达增加84%。额叶皮质5-HT 2A mRNA表达不受激素处理的影响。5-HT 2C受体基因表达在腹侧海马CA 2、腹侧CA 1和下托亚区中被高剂量雌二醇处理降低（降低8-20%）。性腺激素对情绪的影响可以至少部分地通过影响5-HT 2A和5-HT 2C受体表达来介导。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Alterations in female gonadal hormones are associated with anxiety and mood changes. The aim of the present study was to determine influences of chronic gonadal hormone supplementation on 5-HT2A and 5-HT2C receptor mRNA levels in the ventral hippocampus and the frontal cerebral cortex. Ovariectomized adult female Sprague-Dawley rats (n = 37) received implantation of subcutaneous pellets containing different dosages of 17beta-estradiol alone or in combination with progesterone, or placebo pellets, for 2 weeks. Serotonin receptor mRNA levels were analyzed by in situ hybridization in the ventral hippocampus and 5-HT2A receptor mRNA also in the frontal cortex. Estradiol treatment in combination with low-dose progesterone increased 5-HT2A receptor mRNA by 43% in the CA2 region of the ventral hippocampus, while estradiol combined with high-dose progesterone increased the expression of this gene by 84% in ventral CA1. 5-HT2A mRNA expression in the frontal cortex was not influenced by hormone manipulation. 5-HT2C receptor gene expression was in the ventral hippocampus decreased in the CA2, ventral CA1 and the subiculum subregions by high-dose estradiol treatment (8-20% decreases). Effects on mood by gonadal hormones can be mediated, at least partly, through influences on 5-HT2A and 5-HT2C receptor expression. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.