P-31-NMR ANALYSIS OF SYNAPTIC VESICLES - STATUS OF ATP AND INTERNAL PH
P-31-NMR ANALYSIS OF SYNAPTIC VESICLES - STATUS OF ATP AND INTERNAL PH
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DOI：
10.1111/j.1432-1033.1982.tb05817.x
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发表时间：
1982-01-01
期刊：
影响因子：
--
通讯作者：
STADLER, H
中科院分区：
文献类型：
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作者：
FULDNER, HH;STADLER, H
用31 P-NMR研究了条纹电鳐（Torpedo marmoraia）电器胆碱能突触囊泡中ATP的状态。在分离的囊泡中，ATP被发现是唯一主要的含磷酸盐成分。Pγ和Pβ共振的化学位移和线宽与加入悬浮液中的非区室化ATP的化学位移和线宽不同。完整电组织的频谱包含两组ATP共振;其中一个属于囊泡ATP，根据其线宽和化学位移确定。囊泡ATP的光谱特征，线宽和化学位移，可以用交换机制来解释，这也发生在含有乙酰胆碱的模型溶液中，因此，我们认为胆碱能突触囊泡储存ATP和乙酰胆碱以及Mg ~（2+）由于ATP的Pγ共振的化学位移主要由pH以外的因素决定，在这些细胞器的情况下，必须修改其用作内部pH指示剂的概念。
The status of ATP in cholinergic synaptic vesicles from the electric organ ofTorpedo marmoraiahas been studied by31P‐NMR. In isolated vesicles, ATP was found to be the only major phosphate‐containing constituent. The chemical shifts and the linewidths of the Pγand Pβresonances are different from those of uncompartmented ATP added to the suspension. The spectrum of intact electric tissue contains two sets of ATP resonances; one of them belongs to vesicular ATP as identified by its linewidths and chemical shifts.Both spectral characteristics of vesicular ATP, linewidths and chemical shifts, could be explained by an exchange mechanism, which also occurs in model solutions containing acetylcholine, ATP and Mg2+at a pH around 5.5.We therefore conclude that the cholinergic synaptic vesicles store ATP together with acetylcholine and Mg2+essentially in free solution at an acidic pH. Since the chemical shift of the Pγ resonance of ATP is largely determined by factors other than pH, the concept of its use as an indicator for the internal pH has to be modified in the case of these organelles.