A novel strain of lumpy skin disease virus causes clinical disease in cattle in Hong Kong
A novel strain of lumpy skin disease virus causes clinical disease in cattle in Hong Kong
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DOI：
10.1111/tbed.14304
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发表时间：
2021-09-16
影响因子：
4.3
通讯作者：
Beard, Philippa M.
中科院分区：
文献类型：
--
作者：
Flannery, John;Shih, Barbara;Beard, Philippa M.
结节性皮肤病病毒（LSDV）是一种新兴的牛痘病毒病原体，目前正在整个亚洲蔓延。该疾病的情况对亚洲的农民和政策制定者非常重要。2020年10月，香港的野生牛只出现多灶性皮肤结节，与狼疮性皮肤病（LSD）相符。大体和组织病理学进一步支持了诊断，并将样本送至Pirbright研究所的OIE参考实验室进行确认检测。使用定量聚合酶链反应（qPCR）和其他分子分析检测LSDV。这是LSD在香港的首份报告。对LSDV/Hong Kong/2020株进行了全基因组测序（WGS）和系统发育分析，以确定与先前LSD爆发的联系，并更好地了解LSDV出现的驱动因素。对90个核心痘病毒基因的分析表明，LSDV/Hong Kong/2020是一种新毒株，与LSDV的减毒Neethling活疫苗株关系最密切，与非洲、中东和欧洲的野生型LSDV分离株关系较远。对位于痘病毒基因组末端的更多可变区的分析显示，与先前公布的LSDV野生型和疫苗株相比，LSDV/Hong Kong/2020中的基因具有不同的分组模式。这项研究显示，2020年香港爆发的LSD疫情是由一种与2015-2018年中东和欧洲LSD疫情不同的LSDV菌株引起的。在调查LSDV疾病暴发时，强烈建议使用WGS。
Lumpy skin disease virus (LSDV) is an emerging poxviral pathogen of cattle that is currently spreading throughout Asia. The disease situation is of high importance for farmers and policy makers in Asia. In October 2020, feral cattle in Hong Kong developed multi-focal cutaneous nodules consistent with lumpy skin disease (LSD). Gross and histological pathology further supported the diagnosis and samples were sent to the OIE Reference Laboratory at The Pirbright Institute for confirmatory testing. LSDV was detected using quantitative polymerase chain reaction (qPCR) and additional molecular analyses. This is the first report of LSD in Hong Kong. Whole genome sequencing (WGS) of the strain LSDV/Hong Kong/2020 and phylogenetic analysis were carried out in order to identify connections to previous outbreaks of LSD, and better understand the drivers of LSDV emergence. Analysis of the 90 core poxvirus genes revealed LSDV/Hong Kong/2020 was a novel strain most closely related to the live-attenuated Neethling vaccine strains of LSDV and more distantly related to wildtype LSDV isolates from Africa, the Middle East and Europe. Analysis of the more variable regions located towards the termini of the poxvirus genome revealed genes in LSDV/Hong Kong/2020 with different patterns of grouping when compared to previously published wildtype and vaccine strains of LSDV. This work reveals that the LSD outbreak in Hong Kong in 2020 was caused by a different strain of LSDV than the LSD epidemic in the Middle East and Europe in 2015-2018. The use of WGS is highly recommended when investigating LSDV disease outbreaks.