Programmable DNA Nanoswitch Sensing with Solid-State Nanopores
Programmable DNA Nanoswitch Sensing with Solid-State Nanopores
复制标题
DOI：
10.1021/acssensors.9b01053
复制
发表时间：
2019-09-01
期刊：
影响因子：
8.9
通讯作者：
Godin, Michel
中科院分区：
文献类型：
--
作者：
Beamish, Eric;Tabard-Cossa, Vincent;Godin, Michel
固体纳米孔的传感性能受到小分子靶点快速动力学的限制。为了解决这一挑战，我们将小目标的存在转化为长聚合物的大构象变化。在这项工作中，我们探索了固态纳米孔的性能，用于感知利用DNA折纸原理组装的分子纳米开关的构象状态。这些可编程的单分子开关在分子诊断和长期信息存储方面显示出巨大的潜力。我们利用在薄膜上制备的纳米孔研究了线性和环状纳米开关拓扑结构的易位特性，并最终比较了我们的纳米孔平台在检测寨卡病毒生物标志物基因序列中DNA类似物的存在与传统凝胶电泳的性能。我们发现，我们的系统提供了一种高通量的方法，通过使用通常用于纳米孔传感系统的中等带宽的电子设备，仅传感几百个分子，就可以在一个数量级内定量几种目标浓度。
Sensing performance of solid-state nanopores is limited by the fast kinetics of small molecular targets. To address this challenge, we translate the presence of a small target to a large conformational change of a long polymer. In this work, we explore the performance of solid-state nanopores for sensing the conformational states of molecular nanoswitches assembled using the principles of DNA origami. These programmable single-molecule switches show great potential in molecular diagnostics and long-term information storage. We investigate the translocation properties of linear and looped nanoswitch topologies using nanopores fabricated in thin membranes, ultimately comparing the performance of our nanopore platform for detecting the presence of a DNA analogue to a sequence found in a Zika virus biomarker gene with that of conventional gel electrophoresis. We found that our system provides a high-throughput method for quantifying several target concentrations within an order of magnitude by sensing only several hundred molecules using electronics of moderate bandwidth that are conventionally used in nanopore sensing systems.