Evaluating the separation of amphetamines by electrospray ionization ion mobility spectrometry/MS and charge competition within the ESI process.
Evaluating the separation of amphetamines by electrospray ionization ion mobility spectrometry/MS and charge competition within the ESI process.
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通过电喷雾电离离子迁移谱/MS 和 ESI 过程中的电荷竞争评估安非他明的分离。
DOI：
10.1021/ac010858m
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发表时间：
2002
影响因子：
7.4
通讯作者：
HillJr,HerbertH
中科院分区：
文献类型：
--
作者：
Matz,LauraM;HillJr,HerbertH
出于娱乐目的的苯丙胺滥用迅速增加，因此需要快速分析和检测方法。首次用电喷雾质谱联用技术对6种苯丙胺进行了分离，在70位S色谱柱上即可完成完整的分析，比传统的色谱分析技术更快。此外，ESI-IMS/MS对六种化合物的检测下限较低(乙基苯丙胺为15.4 ppb)。在苯丙胺浓度较高的情况下，苯丙胺之间存在电荷竞争。优先电离的程度取决于胺上的官能团。在离子蒸发模型的基础上，对单分析物和双分析物校正曲线进行了评价。测定了6种苯丙胺的蒸发速率，并与选择性电离程度进行了相关分析。对三种典型的ESI溶剂组成的评价表明，添加改性剂(醋酸和甲酸)增强了某些苯丙胺的优先电离程度，并增加了电荷竞争的效果。溶剂研究显示了ESI的复杂性，并为改变分析物之间的电荷竞争提供了可能的策略。总体而言，ESI-IMS/MS是一种很有前途的技术，因为它对水样中的苯丙胺具有灵敏和快速的分离时间；然而，在将其推荐为主流技术之前，需要对生物样品进行进一步的研究。
The rapid increase in amphetamine abuse for recreational purposes has created a need for fast analysis and detection methodologies. For the first time, we show the separation of six amphetamines by ESI-IMS/MS. A complete analysis can be performed in 70 s, which is faster than traditional chromatographic techniques. In addition, ESI-IMS/MS was found to provide low detection limits for the six compounds (15.4 ppb for ethylamphetamine). Charge competition between amphetamines was found to occur at high amphetamine concentrations. The degree of preferential ionization was dependent on the functional group placed on the amine. Both one-analyte and two-analyte calibration curves were evaluated on the basis of the ion evaporation model. Evaporation rates were determined for the six amphetamines, and the rates were correlated with the degree of selective ionization. Evaluation of three typical ESI solvent compositions showed that the addition of a modifier (acetic acid and formic acid) enhanced the degree of preferential ionization for some amphetamines and increased the effect of charge competition. The solvent studies show the complexity of ESI and provide possible strategies for altering the amount of charge competition between analytes. Overall, ESI-IMS/MS appears to be a promising technique because of its sensitivity and rapid separation times for the amphetamines in aqueous samples; however, further research employing biological samples is required before it can be recommended as a mainstream technique.