Multiple hybridization origin of Ranunculus cantoniensis (4x): evidence from trnL-F and ITS sequences and fluorescent in situ hybridization (FISH)
Multiple hybridization origin of Ranunculus cantoniensis (4x): evidence from trnL-F and ITS sequences and fluorescent in situ hybridization (FISH)
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广州毛茛的多重杂交起源 (4x)：来自 trnL-F 和 ITS 序列以及荧光原位杂交 (FISH) 的证据
DOI：
10.1007/s00606-008-0083-2
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发表时间：
2008-11-01
影响因子：
1.9
通讯作者：
Li, Tongjian
中科院分区：
文献类型：
--
作者：
Liao, Liang;Xu, Lingling;Li, Tongjian
广州毛滴虫ITS序列在10个核苷酸位点上呈多态，表明其为异源四倍体。基于该复合体及其近缘种的系统发育分析表明，该复合体的四倍体克隆与R.silerifolius var聚在一起。Silerifolius、R.chinensis、R.Silerifolius var.Dolicasus和R.trionus。叶绿体trnL-F系统发育分析表明，该复合体是一个天然类群，其中四倍体与R.silerifolius var属于同一分支。Dolicatus和R.silerifolius var.Silerifolius，其遗传距离为零。RDNA FISH结果表明，四倍体的rDNA-染色体最大长度与白背天牛相似。独一无二的石斑鱼。结合trnL-F、ITS和FISH数据，推测该四倍体最可能的亲本是Riserifolius var。Silerifolius、中国林蛙(R.chinensis)和林蛙(R.silerifolius var.)Dolicaus，其中白背金藻R.silerifolius var.Silerifolius捐赠最多。DNA序列和染色体FISH的证据表明，该四倍体很可能是同倍体杂种。因此，提出了四倍体形成的方案：四倍体是通过两轮杂交合成的。第一轮是在两对二倍体之间，形成了两个四倍体。第二轮是在两个初级四倍体之间进行的，最终产生了异源四倍体广州根结线虫。
ITS sequences of Ranunculus cantoniensis apparently an allotetraploid were polymorphic at ten nucleotide sites. ITS-based phylogeny of the complex and its allied species showed that ITS clones of the tetraploid were clustered with R. silerifolius var. silerifolius, R. chinensis, R. silerifolius var. dolicathus and R. trigonus. Chloroplast trnL-F phylogeny showed that the complex is a natural group, in which the tetraploid shared the same clade with R. silerifolius var. dolicathus and R. silerifolius var. silerifolius, whose genetic distances were zero. rDNA FISH showed that the longest rDNA-chromosome of the tetraploid was similar to that of R. silerifolius var. dolicathus exclusively. Combining trnL-F, ITS and FISH data, it is suggested that the most probable parents of the tetraploid were R. silerifolius var. silerifolius, R. chinensis and R. silerifolius var. dolicathus, among them R. silerifolius var. silerifolius donated most. Evidences from DNA sequences and chromosome FISH indicated that the tetraploid was most probably a homoploid hybrid. Thus, a scenario of the tetraploid formation is proposed: the tetraploid was synthesized by two rounds of hybridization. The first round was between two pairs of diploids, forming two tetraploids. The second round was between the two primary tetraploids, producing the allotetraploid, R. cantoniensis, eventually.