Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas
Cytometrical image analysis for immunohistochemical hormone receptor status in breast carcinomas
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DOI：
10.1046/j.1440-1827.2003.01547.x
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发表时间：
2003-10-01
影响因子：
2.2
通讯作者：
Tani, Y
中科院分区：
文献类型：
--
作者：
Hatanaka, Y;Hashizume, K;Tani, Y
应用WinROOF软件建立了细胞核蛋白质图像分析方法，用于雌激素受体和孕激素受体的检测。分别使用抗ER（克隆1D 5）和抗PR（克隆PgR 636）与右旋糖酐聚合物试剂EnVision+（均在日本获批用于体外诊断）进行雌激素受体（ER）和孕激素受体（PR）的免疫组织化学评价。将免疫染色结果在Windows中捕获为数字图像，然后在WinROOF中用宏指令进行分析，用于分析每个捕获的区域，用色原免疫标记或用苏木精复染。该图像分析方法根据染色强度对癌细胞的免疫染色细胞核进行分级，并计算ER和PR的标记指数（LI）。此外，当对20例乳腺癌进行定量时，LI与组织学评分（HSCORE）的结果高度相关。关于ER，当LI中的20%被认为是阳性的截止点时，ER在计算机辅助分析中的阳性率为75%（15/20例），并且与基于HSCORE的分析结果完全一致。这些结果表明，基于细胞图像分析的定量可以适当地应用于ER和PR的免疫组化状态的客观测定。
A cytometrical image analyzing method for nuclear protein was established using WinROOF, a commercially available, inexpensive software, to determine the status of both estrogen and progesterone receptors. Immunohistochemical evaluation of estrogen receptors (ER) and progesterone receptors (PR) was performed with the anti-ER (clone 1D5) and the anti-PR (clone PgR636), respectively, combined with dextran polymer reagent EnVision+, all of which are approved in vitro diagnostics in Japan. The immunostained results were captured as digital images in Windows, and then analyzed in WinROOF with macroinstructions for analyzing each captured area either immunolabeled with chromogen or counterstained with hematoxylin. This image analysis method graded the immunostained nuclei of carcinoma cells based on staining intensities, and calculated the labeling index (LI) for both ER and PR. Furthermore, the LI correlated highly with the results from a histology score (HSCORE) when 20 breast carcinomas were quantified. Regarding ER, when 20% in the LI was considered as the cut-off point for positive, the positivity of ER in computer-assisted analysis was 75% (15 of 20 cases), and was completely concordant with that of HSCORE-based analysis. These results indicate that the cytometrical image analysis-based quantification could be appropriately applied to the objective determination of the immunohistochemical status of both ER and PR.