4-vinylphenol-induced pneumotoxicity and hepatotoxicity in mice
4-vinylphenol-induced pneumotoxicity and hepatotoxicity in mice
复制标题
DOI：
10.1080/01926230290105866
复制
发表时间：
2002-09-01
影响因子：
1.5
通讯作者：
Snyder, PW
中科院分区：
文献类型：
--
作者：
Carlson, GP;Ullman, M;Snyder, PW
4-乙烯基酚（4-羟基苯乙烯，4-乙烯基酚，4-VP）天然存在于一些食品中，并被用作食品中的调味剂。它被用于合成聚合物和树脂的生产。据报道，它也是大鼠和人体内苯乙烯的一种微量代谢物。给小鼠注射不同剂量的4-乙烯基酚。通过测定血清山梨醇脱氢酶（SDH）和光镜观察肝毒性。通过测量支气管肺泡灌洗液（BALF）中的蛋白质、细胞和乳酸脱氢酶活性以及光镜来评估肺毒性。4-VP引起剂量依赖性血清SDH升高和轻度肝细胞肿胀。引起BALF细胞数量和乳酸脱氢酶活性的增加。显微镜下，细支气管在12小时内广泛且严重坏死。48小时后细支气管明显再上皮化。这些研究表明，4-乙烯基酚具有肝毒性和肺毒性。
4-Vinylphenol (4-hydroxystyrene, 4-ethenylphenol, 4-VP) occurs naturally in some foods and has been used as a flavoring agent in food products. It is used synthetically in the production of polymers and resins. It has also been reported to be a minor metabolite of styrene in rats and humans. Varying doses of 4-vinylphenol were administered ip to mice. Hepatotoxicity was assessed by measuring serum sorbitol dehydrogenase (SDH) and by light microscopy. Pneumotoxicity was assessed by measuring proteins, cells, and lactate dehydrogenase activity in bronchoalveolar lavage fluid (BALF) and by light microscopy. 4-VP caused a dose-dependent increase in serum SDH and mild hepatocellular swelling. It caused an increase in cell number and lactate dehydrogenase activity in BALF. Microscopically, there was widespread and severe necrosis of the bronchioles by 12 hours. Re-epithelialzation of the bronchioles was evident by 48 hours. These studies indicate that 4-vinylphenol is both hepatotoxic and pneumotoxic.