Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25
Construction of a Single Polypeptide that Matures and Exports the Lasso Peptide Microcin J25
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DOI：
10.1002/cbic.201100596
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发表时间：
2012-02-13
期刊：
影响因子：
3.2
通讯作者：
Link, A. James
中科院分区：
文献类型：
--
作者：
Pan, Si Jia;Rajniak, Jakub;Link, A. James
套索肽微霉素J25 （MccJ25）由两种酶成熟，并通过假定的ABC转运体输出。我们利用诱变技术探讨了成熟酶的功能。我们证明了这些酶与中间连接体的融合可以产生MccJ25。即使ABC转运体和这两种酶之间的151 kDa的三方融合也能够产生和输出MccJ25。
Roped in: The lasso peptide microcin J25 (MccJ25) is matured by two enzymes and is exported by a putative ABC transporter. We probed the function of the maturation enzymes using mutagenesis. We demonstrate that fusions of the enzymes with intervening linkers can produce MccJ25. Even a 151 kDa tripartite fusion between the ABC transporter and the two enzymes is capable of producing and exporting MccJ25.