EVIDENCE FOR THE IDENTITY OF GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE AND CLASS-III ALCOHOL-DEHYDROGENASE
EVIDENCE FOR THE IDENTITY OF GLUTATHIONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE AND CLASS-III ALCOHOL-DEHYDROGENASE
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DOI：
10.1016/0014-5793(89)81797-1
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发表时间：
1989-10-23
期刊：
影响因子：
3.5
通讯作者：
UOTILA, L
中科院分区：
文献类型：
--
作者：
KOIVUSALO, M;BAUMANN, M;UOTILA, L
甲醛脱氢酶（EC 1.2.1.1）是一种广泛存在的酶，它催化由甲醛和谷胱甘肽形成的s -羟甲基谷胱甘肽在NAD存在下氧化为s -甲酰基谷胱甘肽。我们测定了电泳均相的大鼠肝脏甲醛脱氢酶的5个色氨酸肽（共57个氨基酸）的氨基酸序列，发现它们都与大鼠肝脏III类乙醇脱氢酶（ADH-2）的序列完全同源。甲醛脱氢酶被发现能够在高pH值下催化长链脂肪醇-比辛醇和12-羟基十二酸钠依赖于nad的氧化，但乙醇仅在非常高的底物浓度下使用，吡唑没有抑制作用。氨基酸序列同源性和相同的结构及动力学性质表明甲醛脱氢酶与哺乳动物III类醇脱氢酶是相同的酶。甲醛脱氢酶;III类醇脱氢酶；序列同源性;氨基酸序列
Formaldehyde dehydrogenase (EC 1.2.1.1) is a widely occurring enzyme which catalyzes the oxidation ofS-hydroxymethylglutathione, formed from formaldehyde and glutathione, intoS-formylglutathione in the presence of NAD. We determined the amino acid sequences for 5 tryptic peptides (containing altogether 57 amino acids) from electrophoretically homogeneous rat liver formaldehyde dehydrogenase and found that they all were exactly homologous to the sequence of rat liver class III alcohol dehydrogenase (ADH-2). Formaldehyde dehydrogenase was found to be able at high pH values to catalyze the NAD-dependent oxidation of long-chain aliphatic alcohols liken-octanol and 12-hydroxydodecanoate but ethanol was used only at very high substrate concentrations and pyrazole was not inhibitory. The amino acid sequence homology and identical structural and kinetic properties indicate that formaldehyde dehydrogenase and the mammalian class III alcohol dehydrogenases are identical enzymes.Formaldehyde dehydrogenase; Alcohol dehydrogenase, class III; Sequence homology; Amino acid sequence