Translational Landscape of a C4 Plant, <i>Sorghum bicolor</i>, Under Normal and Sulfur-Deficient Conditions
Translational Landscape of a C4 Plant, <i>Sorghum bicolor</i>, Under Normal and Sulfur-Deficient Conditions
复制标题
C4 植物<i>高粱</i>在正常和缺硫条件下的转化景观
DOI：
10.1093/pcp/pcac023
复制
发表时间：
2022
影响因子：
4.9
通讯作者：
Fujiwara Toru
中科院分区：
文献类型：
--
作者：
Sotta Naoyuki;Chiba Yukako;Aoyama Haruka;Takamatsu Seidai;Suzuki Takamasa;Miwa Kyoko;Yamashita Yui;Naito Satoshi;Fujiwara Toru
最近积累的基因组和转录组信息促进了遗传学研究。越来越多的证据表明，翻译是一个重要的调控步骤，转录组并不一定反映功能蛋白质生产的概况。核糖体保护的mRNA片段的深度测序（核糖体分析或Ribo-seq）已经能够进行全基因组翻译分析。高粱是一种重要的C4谷物，不仅是粮食，而且是饲料和生物能源资源。它对恶劣环境的抵抗力使其成为农业上重要的研究课题。然而，高粱的全基因组翻译谱仍然缺失。在这项研究中，我们利用Ribo-seq并在整个基因组中鉴定了活跃翻译的阅读框架。我们检测了高粱参考基因组版本3.1中注释的4，843个主要开放阅读框（ORF）的翻译，并揭示了许多未注释的翻译事件。正常和缺硫条件下生长的高粱之间的转录组和翻译组的比较显示，基因表达在转录和翻译水平独立调节。我们的研究揭示了高粱对硫的反应的翻译景观，并提供了可以作为扩展高粱遗传研究（包括翻译调控研究）的基本资源的数据集。
Recent accumulation of genomic and transcriptomic information has facilitated genetic studies. Increasing evidence has demonstrated that translation is an important regulatory step, and the transcriptome does not necessarily reflect the profile of functional protein production. Deep sequencing of ribosome-protected mRNA fragments (ribosome profiling or Ribo-seq) has enabled genome-wide analysis of translation. Sorghum is a C4cereal important not only as food but also as forage and a bioenergy resource. Its resistance to harsh environments has made it an agriculturally important research subject. Yet genome-wide translational profiles in sorghum are still missing. In this study, we took advantage of Ribo-seq and identified actively translated reading frames throughout the genome. We detected translation of 4,843 main open reading frames (ORFs) annotated in the sorghum reference genome version 3.1 and revealed a number of unannotated translational events. A comparison of the transcriptome and translatome between sorghums grown under normal and sulfur-deficient conditions revealed that gene expression is modulated independently at transcript and translation levels. Our study revealed the translational landscape of sorghum’s response to sulfur and provides datasets that could serve as a fundamental resource to extend genetic research on sorghum, including studies on translational regulation.