Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases
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血清中 microRNA 的表征：一类用于诊断癌症和其他疾病的新型生物标志物
DOI：
10.1038/cr.2008.282
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发表时间：
2008-10-01
期刊：
影响因子：
44.1
通讯作者：
Zhang, Chen-Yu
中科院分区：
文献类型：
--
作者：
Chen, Xi;Ba, Yi;Zhang, Chen-Yu
MicroRNAs(MiRNAs)在各种组织中的异常表达与包括癌症在内的多种疾病有关。在这里，我们证明了miRNAs存在于人类和其他动物(如小鼠、大鼠、牛胎、小牛和马)的血清和血浆中。血清中miRNAs的水平是稳定的，可重复性的，并且在同一物种的个体之间是一致的。利用Solexa软件对中国健康受试者的所有血清miRNAs进行测序，发现男性和女性的血清miRNAs分别超过100和91个。我们还确定了肺癌、结直肠癌和糖尿病患者血清miRNAs的特定表达模式，为血清miRNAs包含各种疾病的指纹提供了证据。Solexa获得的两种非小细胞肺癌特异性血清miRNAs在75名健康捐赠者和152名癌症患者的独立试验中进一步得到验证，采用定量逆转录聚合酶链式反应分析。通过这些分析，我们得出结论，血清miRNAs可以作为检测各种癌症和其他疾病的潜在生物标志物。
Dysregulated expression of microRNAs (miRNAs) in various tissues has been associated with a variety of diseases, including cancers. Here we demonstrate that miRNAs are present in the serum and plasma of humans and other animals such as mice, rats, bovine fetuses, calves, and horses. The levels of miRNAs in serum are stable, reproducible, and consistent among individuals of the same species. Employing Solexa, we sequenced all serum miRNAs of healthy Chinese subjects and found over 100 and 91 serum miRNAs in male and female subjects, respectively. We also identified specific expression patterns of serum miRNAs for lung cancer, colorectal cancer, and diabetes, providing evidence that serum miRNAs contain fingerprints for various diseases. Two non-small cell lung cancer-specific serum miRNAs obtained by Solexa were further validated in an independent trial of 75 healthy donors and 152 cancer patients, using quantitative reverse transcription polymerase chain reaction assays. Through these analyses, we conclude that serum miRNAs can serve as potential biomarkers for the detection of various cancers and other diseases.