THE EFFECTS OF VARIOUS ANTIDEPRESSANT DRUGS ON THE FINE-STRUCTURE OF NEURONS OF THE CINGULATE CORTEX IN CULTURE
THE EFFECTS OF VARIOUS ANTIDEPRESSANT DRUGS ON THE FINE-STRUCTURE OF NEURONS OF THE CINGULATE CORTEX IN CULTURE
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DOI：
10.1016/0306-4522(90)90099-p
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
BIRD, MM
中科院分区：
文献类型：
--
作者：
BALKLARA, A;BIRD, MM
我们在体外研究了抗抑郁药物对扣带回神经元和突触的形态和精细结构的影响。分离的16天胎鼠的细胞培养在阿米替林和地昔帕明(三环类抗抑郁药)、米安色林和西酞普兰(非三环类抗抑郁药)存在或不加药物的情况下维持3周。与对照组相比，阿米替林和地塞帕明处理组的突触显示出更多的突触前囊泡和更广泛的膜特化，以及突触前和突触后包被囊泡的数量增加。在米安色林和西酞普兰处理的培养中，突触特化的长度增加，并在轴突底部观察到线粒体聚集。我们的结果表明，长期接触抗抑郁药物会导致细胞内结构的显著变化，包括突触超微结构的变化。
We have examined the effects of antidepressant drugs on the morphology and fine-structure of cingulate cortex neurons and synapses in vitro. Dissociated cell cultures from 16-day-old rat fetuses were maintained for up to three weeks in the presence of amitriptyline and desipramine (tricyclic antidepressants) mianserin and citalopram (non-tricyclic antidepressants) or without drugs as controls. Synapses in cultures exposed to amitriptyline and desipramine displayed increased numbers of presynaptic vesicles and more extensive membrane specializations than in control cultures, and increased numbers of coated vesicles both pre- and postsynaptically. In mianserin- and citalopram-treated cultures, synaptic specializations were increased in length, and aggregates of mitochondria at the base of neurites were observed. Our results indicate that long-term exposure to antidepressant drugs results in significant changes in intracellular structure, including changes in synaptic ultrastructure.