Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics.
Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics.
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淋巴瘤来源细胞系的表征：EB 病毒核抗原阳性和阴性细胞系的比较。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
L. Novikovs
中科院分区：
文献类型：
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作者：
I. Magrath;P. Pizzo;J. Whang‐Peng;E. Douglass;O. Alabaster;P. Gerber;C. Freeman;L. Novikovs
16个淋巴样细胞系来自伯基特或非伯基特型未分化淋巴瘤患者。它们直接从肿瘤活检、浆液性渗出液或骨髓中获得。在10个细胞系中，EB病毒（EBV）核抗原（EBNA）检测不到;其余6个细胞系为EBNA阳性（EB-pos）。在16个品系中，15个为非整倍体，具有可检测到的染色体14 q+标记（11个具有+8 14易位）。这15个细胞系，包括EBNA阴性（EB-neg）细胞系，被认为是肿瘤细胞来源。剩余的细胞系主要由来自正常淋巴细胞的二倍体细胞组成，但也存在一些肿瘤来源的细胞。4个来源于EB-neg肿瘤的EB-pos细胞系具有与肿瘤细胞来源一致的非整倍体核型（包括2个中的8号;14号易位），这表明肿瘤细胞在体外被EBV感染或存在于体内的极小部分EB-pos肿瘤细胞（或潜在肿瘤细胞）产生了该细胞系的优势细胞。从未分化淋巴瘤建立的EB-neg B细胞系和EB-pos细胞系差异很大。EB-neg线始终较小的电子平均细胞体积和窄角光散射比EB-pos线。这一发现与EB-pos系中较低的核质比相关。在标准培养条件下，EB-neg系也比EB-pos系具有更高的饱和细胞密度。数据表明，要么EB病毒影响淋巴细胞系的形态和生理特征，要么EB-neg B细胞系和EB-pos细胞系最终源自不同的淋巴细胞亚群，或者两者都可能适用。
Sixteen lymphoid cell lines were derived from patients with undifferentiated lymphoma of Burkitt's or non-Burkitt's type. They were obtained directly from tumor biopsies, from serous effusions, or from bone marrow. In 10 of the cell lines, the Epstein-Barr virus (EBV) nuclear antigen (EBNA) was undetectable; the remaining 6 lines were EBNA-positive (EB-pos). Of the 16 lines, 15 were aneuploid, with detectable chromosome "14q+ markers (11 had +8;14 translocations). These 15 lines, which included the EBNA-negative (EB-neg) lines, were believed to be of tumor cell origin. The remaining line consisted predominantly of diploid cells derived from normal lymphocytes, but some cells of tumor origin were present. Four EB-pos cell lines derived from EB-neg tumors had an aneuploid karyotype consistent with an origin from tumor cells (including no.8;14 translocation in two), which suggested that either tumor cells were infected with EBV in vitro or a tiny fraction of EB-pos tumor cells (or potential tumor cells) present in vivo gave rise to the predominant cell of the line. EB-neg B-cell lines and EB-pos cell lines established from undifferentiated lymphomas differed greatly. EB-neg lines had consistently smaller electronic mean cell volumes and narrow-angle light scatter than did EB-pos lines. This finding correlated with a lower nuclear:cytoplasmic ratio in EB-pos lines. EB-neg lines also had higher saturation cell densities than did EB-pos lines under standard culture conditions. The data indicate either that EBV influences the morphologic and physiologic characteristics of lymphoid cell lines or that EB-neg B-cell lines and EB-pos cell lines are derived ultimately from different lymphocyte subpopulations or that both may apply.