Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary metabolites of atrazine, malathion, and 2,4-dichlorophenoxyacetic acid
Isotope dilution high-performance liquid chromatography/tandem mass spectrometry method for quantifying urinary metabolites of atrazine, malathion, and 2,4-dichlorophenoxyacetic acid
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DOI：
10.1021/ac990130u
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发表时间：
1999-08-15
影响因子：
7.4
通讯作者：
Barr, DB
中科院分区：
文献类型：
--
作者：
Beeson, MD;Driskell, WJ;Barr, DB
建立了一种同位素稀释高效液相色谱/串联质谱（HPLC/MS/MS）联用技术，用于农药阿特拉津、马拉硫磷和2，4-二氯苯氧乙酸（2，4-D）在尿液中代谢产物的定量分析。用有机溶剂提取尿样，并浓缩有机部分。然后使用HPLC/MS/MS分析浓缩物。代谢物的检测限小于0.5 μ g/L（十亿分之一），在10 mL尿液中，具有高度的准确度和精密度。
We have developed an isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for quantifying the urinary metabolites of the pesticides atrazine, malathion, and 2,4-dichlorophenoxyacetic acid (2,4-D). Urine samples are extracted with an organic solvent, and the organic fraction is concentrated. The concentrate is then analyzed using HPLC/MS/MS. The limits of detection for the metabolites are less than 0.5 mu g/L (parts per billion) in 10 mL of urine, with a high degree of accuracy and precision.