ALTERNATIVE PATHWAY ACTIVATION OF T-CELLS BY BINDING OF CD2 TO ITS CELL-SURFACE LIGAND
ALTERNATIVE PATHWAY ACTIVATION OF T-CELLS BY BINDING OF CD2 TO ITS CELL-SURFACE LIGAND
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DOI：
10.1038/326298a0
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发表时间：
1987-03-19
期刊：
影响因子：
64.8
通讯作者：
MEUER, SC
中科院分区：
文献类型：
--
作者：
HUNIG, T;TIEFENTHALER, G;MEUER, SC
静息T淋巴细胞的激活是由细胞表面受体与其相应配体的相互作用引发的。除了通过CD 3（T3）-Ti抗原受体复合物1激活外，最近的实验还证明了通过CD 2（T11）分子2 -4（传统上称为红细胞（E）受体）诱导T细胞增殖，T细胞可以通过该分子结合红细胞（RBC）5-7。这种T细胞活化的“替代途径”2在体外对与CD 2不同表位结合的抗CD 2单克隆抗体（mAb）组合（如抗T112 + T113的mAb）的应答反应中得到证实（参考文献2）。该活化途径的生理重要性只能通过研究天然存在的CD 2配体对T细胞活化的影响来评估。我们最近描述了这样一种配体，一种表观相对分子质量为42，000（Mr 42 K）的糖蛋白，在所有血细胞和一些其他组织上表达9，11。在这里，我们证明了这种细胞表面分子，称为T11靶结构或T11 TS，CD 2（T11）的结合诱导静息T细胞的反应性，由单克隆抗体T113决定簇或亚促有丝分裂浓度的抗T112 + 3单克隆抗体的促有丝分裂刺激。因此，通过旁路途径活化T细胞所需的信号之一由CD 2与天然存在的互补细胞表面分子的相互作用提供。
Activation of resting T lymphocytes is initiated by the interaction of cell-surface receptors with their corresponding ligands. In addition to activation through the CD3 (T3)-Ti antigen-receptor complex1, recent experiments have demonstrated induction of T-cell proliferation through the CD2 (T11) molecule2–4, traditionally known as the erythrocyte(E)-receptor, through which T cells can bind red blood cells (RBC)5–7. This 'alternative pathway' of T-cell activation2was observedin vitroin response to combinations of anti-CD2 monoclonal antibodies (mAbs) that bind to distinct epitopes of CD2, such as mAbs against T112plus T113(ref. 2). The physiological importance of this activation pathway can be assessed only by studying the effects of a naturally occurring ligand of CD2 on T-cell activation. We have recently described such a ligand, a glycoprotein of apparent relative molecular mass 42,000 (Mr42K) that is expressed on all blood cells and some other tissues9,11. Here we demonstrate that binding of this cell surface molecule, termed T11 target structure or T11TS, to CD2 (T11) induces reactivity in resting T cells to a mitogenic stimulus given by a mAb to the T113determinant or by submitogenic concentrations of anti-T112+3mAbs. Thus, one of the signals required for T-cell activation through the alternative pathway is provided by the interaction of CD2 with a naturally occurring complementary cell-surface molecule.