Effects of crustacean cardioactive peptide on the hearts of two Orthopteran insects, and the demonstration of a Frank-Starling-like effect
Effects of crustacean cardioactive peptide on the hearts of two Orthopteran insects, and the demonstration of a Frank-Starling-like effect
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DOI：
10.1016/j.ygcen.2011.01.015
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发表时间：
2011-04-01
影响因子：
2.7
通讯作者：
Lange, Angela B.
中科院分区：
文献类型：
--
作者：
da Silva, Sara R.;da Silva, Rosa;Lange, Angela B.
与脊椎动物的心血管系统一样，直翅目昆虫齿外芽孢杆菌和飞蝗的背侧血管也通过神经递质、神经调质和神经激素的作用受到肌源性和神经性的控制。以前的研究表明，兴奋性神经肽，甲壳动物心脏活性肽（CCAP），触发心率增加在B。在许多昆虫中，CCAP样免疫反应性存在于心脏的神经支配中。在本研究中，CCAP导致B组心率和血淋巴流速或心输出量呈剂量依赖性增加。齿外相反，CCAP导致L migratoria的每搏输出量和心输出量显著增加，而不改变心率或主动脉收缩频率。血淋巴常从流出口流出。随着CCAP浓度的增加，后主动脉和前心脏上的小开口或瓣膜的移行受到抑制，在10（-7）M CCAP时观察到完全抑制。在蝗虫中，CCAP通过同步关闭流出口来增加背侧血管中的血淋巴体积，从而导致更有力的心脏收缩以及增加的每搏输出量和心输出量，而不通过类似于脊椎动物中的Frank-Starling机制的生理机制来改变心率。因此，甲壳动物心脏活性肽改变了B中心脏组织的收缩特性。extradentatum和L. migratoria，允许增加血液流动和循环。(C)2011 Elsevier Inc. All rights reserved.
Like vertebrate cardiovascular systems, the dorsal vessel of the Orthopteran insects Baculum extradentatum and Locusta migratoria is under myogenic as well as neural control, through the action of neurotransmitters, neuromodulators and neurohormones. It was previously shown that the excitatory neuropeptide, crustacean cardioactive peptide (CCAP), triggers an increase in heart rate in B. extradentatum, and CCAP-like immunoreactivity is present in the innervation to the heart in many insects. In the present study, CCAP resulted in a dose-dependent increase in heart rate and hemolymph flow velocity, or cardiac output, in B. extradentatum. In contrast, CCAP led to a significant increase in stroke volume and cardiac output in L migratoria without modifying heart rate or aortic contraction frequency. Hemolymph flow through the excurrent ostia oft. migratoria, small openings or valves on the posterior aorta and anterior heart, was inhibited with increasing concentrations of CCAP, with complete inhibition seen at 10(-7) M CCAP. In the locust, CCAP increases the volume of hemolymph in the dorsal vessel by the synchronous closing of the excurrent ostia, resulting in more forceful heart contractions and increased stroke volume and cardiac output, without modifying heart rate through a physiological mechanism analogous to the Frank-Starling mechanism in vertebrates. Therefore, crustacean cardioactive peptide alters the contractile properties of cardiac tissue in both B. extradentatum and L. migratoria, allowing for an increase in blood flow and circulation. (C) 2011 Elsevier Inc. All rights reserved.