Case report: two cases of mature oocytes found in prepubertal girls during ovarian tissue cryopreservation.
Case report: two cases of mature oocytes found in prepubertal girls during ovarian tissue cryopreservation.
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DOI：
10.1016/j.xfre.2021.03.007
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Maher JY
中科院分区：
文献类型：
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作者：
Hanfling SN;Parikh T;Mayhew A;Robinson E;Graham J;Gomez-Lobo V;Maher JY
目的报道2例青春期前女孩卵巢组织冷冻保存(OTC)后发现的成熟卵母细胞。案件报告。大型三级保健儿童医院和一家私人生育诊所。一名患有β-地中海贫血的8岁青春期前女孩和一名患有镰状细胞疾病的2岁女孩，在骨髓移植前都进行了非处方药治疗。每例患者均行腹腔镜下右侧卵巢切除术。对卵巢皮质组织进行缓慢冷冻和长期保存，然后对所有卵母细胞进行玻璃化冷冻。在非处方药处理时发现的卵母细胞，用于保存生育能力。在完全切除右侧卵巢后，每例患者在组织处理过程中均发现1个成熟的中期II期卵母细胞。两名患者都还没有回来使用组织或卵母细胞。据我们所知，这是在青春期前非处方药处理过程中发现的第一个成熟卵母细胞。这些发现可能表明有必要加强对青春期前卵母细胞发育的研究，并建议在OTC时检查成熟和未成熟卵母细胞的培养液的技术应该得到更广泛的推广，并可能推荐在青春期前患者中使用，以优化生育能力保存方法。
To report two cases of mature oocytes found in prepubertal girls undergoing ovarian tissue cryopreservation (OTC). Case report. Large tertiary care children’s hospital and a private fertility clinic. An 8-year-old prepubertal girl with β-thalassemia and a 2-year-old girl with sickle cell disease who both underwent OTC before bone marrow transplantations. Laparoscopic right oophorectomy was performed in each patient. The ovarian cortical tissue was processed for slow freezing and long-term storage, and all oocytes were subsequently vitrified. Oocytes found at the time of OTC processing for fertility preservation. After a complete right oophorectomy, one mature metaphase II oocyte was discovered on tissue processing for OTC in each patient. Neither patient has yet returned for use of tissue or oocytes. To our knowledge, this is the first report of mature oocytes found during prepubertal OTC processing. These findings may indicate the need for increased research regarding prepubertal oocyte development and suggest that the technique of examining the media for both mature and immature oocytes at the time of OTC should become more widespread and perhaps recommended in prepubertal patients to optimize fertility preservation methods.