Reduction of experimental Salmonella and Campylobacter contamination of chicken skin by application of lytic bacteriophages
Reduction of experimental Salmonella and Campylobacter contamination of chicken skin by application of lytic bacteriophages
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DOI：
10.1128/aem.69.8.5032-5036.2003
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发表时间：
2003-08-01
影响因子：
4.4
通讯作者：
Barrow, PA
中科院分区：
文献类型：
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作者：
Goode, D;Allen, VM;Barrow, PA
将裂解噬菌体应用于被肠炎沙门氏菌或空肠弯曲菌以1的感染复数(MOI)污染的鸡皮，可提高滴度，使病原菌数量减少不到1log(10)个单位。MOI为100~1000的噬菌体在48小时内迅速将可回收的细菌数量减少了2log(10)个单位。当沙门氏菌污染水平较低(单位皮肤面积的对数为2)且MOI为10(5)时，没有生物被回收。通过增加应用的噬菌体颗粒数量(即MOI为10(7))，也可以消除由于限制而表现出高抗药性但噬菌体能够附着的其他沙门氏菌菌株。
Lytic bacteriophages, applied to chicken skin that had been experimentally contaminated with Salmonella enterica serovar Enteritidis or Campylobacter jejuni at a multiplicity of infection (MOI) of 1, increased in titer and reduced the pathogen numbers by less than 1 log(10) unit. Phages applied at a MOI of 100 to 1,000 rapidly reduced the recoverable bacterial numbers by up to 2 log(10) units over 48 h. When the level of Salmonella contamination was low (< log(10) 2 per unit area of skin) and the MOI was 10(5), no organisms were recovered. By increasing the number of phage particles applied (i.e., MOI of 10(7)), it was also possible to eliminate other Salmonella strains that showed high levels of resistance because of restriction but to which the phages were able to attach.