A SENSORY ROLE FOR NEURONAL GROWTH CONE FILOPODIA
A SENSORY ROLE FOR NEURONAL GROWTH CONE FILOPODIA
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DOI：
10.1038/361721a0
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发表时间：
1993-02-25
期刊：
影响因子：
64.8
通讯作者：
KATER, SB
中科院分区：
文献类型：
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作者：
DAVENPORT, RW;DOU, P;KATER, SB
神经元生长锥丝状伪足的动态性质导致丝状伪足的主要功能是对其直接环境进行采样1 -3，响应并转导影响生长锥行为4 -6和形状7 -11的环境信号。丝状伪足似乎非常适合充当类似天线的传感器，它们的宽阔跨度允许在大大增强的半径内进行信息采样，并且向前投射的丝状伪足早在前进的生长锥本身之前就在分子地形中遇到了潜在的线索12。培养中的丝状伪足可以发挥结构作用13，施加机械张力14 -16并选择性地粘附于其周围17 -20。丝状伪足是否具有一般的感觉作用还没有被直接测试，主要是因为它们的小尺寸，这限制了电生理学方法，以及它们与母生长锥的整体关系，这阻止了它们不同功能的分辨率。在这里，我们使用外科手术分离个别丝状伪足从他们的父母生长锥，并通过监测其形态和钙第二信使系统，我们表明，神经元生长锥丝状伪足包含信号转导机制，允许自主反应和远程环境信息传输到他们的父母生长锥。
THE dynamic nature of neuronal growth cone filopodia led to the suggestion that the primary function of filopodia is to sample their immediate environment1-3, responding to and transducing environmental signals that affect growth cone behaviour4-6 and shape7-11. Filopodia seem well suited to serve as antenna-like sensors, their broad span allows sampling of information over a greatly enhanced radius, and forward-projecting filopodia encounter potential cues in the molecular terrain long before the advancing growth cone itself12. Filopodia in culture can serve structural roles13, exert mechanical tension14-16 and selectively adhere to their surrounding17-20. Whether or not filopodia have a general sensory role has not been tested directly, largely because of their small size, which limits an electrophysiological approach, and their integral relationship with the parent growth cone, which prevents resolution of their different functions. Here we use surgical procedures to isolate individual filopodia from their parent growth cone and, by monitoring their morphology and calcium second messenger systems, we show that neuronal growth cone filopodia contain signal transduction mechanisms that allow autonomous responses and the transmission of distant environmental information to their parent growth cone.